克霉唑对髓系白血病细胞增殖影响的研究  

Effect of clotrimazole on proliferation of myeloid leukemia cells

作　　者：赵秀峰[1,2] 王巍[1] 刘志宇[1] 周晋[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院血液内科,黑龙江哈尔滨150001 [2]牡丹江医学院红旗医院肿瘤科,黑龙江牡丹江157011

出　　处：《哈尔滨医科大学学报》2010年第5期433-435,共3页Journal of Harbin Medical University

基　　金：黑龙江省青年科学基金项目(Q00C002);黑龙江省教育厅科研课题(11521186);哈尔滨市科技创新人才研究资金(2008RFQXS100)

摘　　要：目的研究抗真菌药物克霉唑作为中电导钙激活性钾通道阻断剂对髓系白血病细胞增殖的影响。方法通过台盼蓝拒染计数法检测克霉唑对髓系白血病细胞株K562和HL-60生存活力的影响,通过流式细胞仪检测细胞周期变化。结果克霉唑以浓度依赖方式显著抑制K562和HL-60细胞生长。克霉唑15μmol/L作用48 h后K562与HL-60细胞活力明显下降,分别为(70.7±2.6)%、(75.1±3.9)%,而骨髓基质细胞活力为(88.3±2.1)%,没有明显毒性;G0/G1期分别为(50.8±1.9)%、(65.6±3.0)%,与对照组(37.6±1.5)%、(43.9±2.3)%相比具有显著性差异(P<0.01),细胞周期停滞于G0/G1期。结论钙激活性钾通道阻断剂克霉唑能够抑制髓系白血病细胞株K562和HL-60细胞生长,使细胞周期阻滞于G0/G1期。Objective To investigate the effect of the antifungal agent clotrimazole,an inhibitor of the intermediate-conductance Ca2＋-activated K＋（IK） channel,on proliferation of myeloid leukemia cells.Methods The effects of clotrimazole on cells viability of K562 and HL-60 cell lines were detected by trypan blue dye exclusion method.Cell cycle distribution was examined by flow cytometry.Results Cell viability was suppressed by clotrimazole in a dose-dependent manner in K562 and HL-60 cell lines.After 48 h,the median cell viability of 15 μmol/L clotrimazole treatment was determined as follows for each cells：（70.7±2.6）% in K562,（75.1±3.9）% in HL-60 and（88.3±2.1）% in bone marrow stromal cells,whereas no appreciable toxicity was observed in bone marrow stromal cells.DNA histograms showed a significantly larger percentage of cells in G0/G1phase（50.8±1.9）% in K562 and（65.6±3.0）% in HL-60 than that detected in the control group（37.6±1.5）% in K562 and（43.9±2.3）% in HL-60（P0.01）.Conclusion Clotrimazole,an inhibitor of intermediate-conductance Ca2＋-activated K＋ channel,inhibits proliferation of K562 and HL-60 cell lines,and arrests the cell cycle in G0/G1 phase.

关 键 词：克霉唑 增殖 白血病 K562 HL-60 

分 类 号：R733.71[医药卫生—肿瘤]