电针对局灶脑缺血再灌注大鼠大脑皮质胎盘生长因子及其受体Flt-1 mRNA和蛋白表达的影响及其促进脑内血管再生的作用  

The Effects of Electroacupuncture on mRNA and Protein Expression of Placental Growth Factor and Flt-1 and Promotes Revascularization in the Brain After Focal Cerebral Ischemia/Reperfusion in SD Rats

作　　者：武磊[1] 罗勇[1] 张珊珊[1] 

机构地区：[1]重庆医科大学附属第一医院神经内科重庆市神经病学重点实验室,重庆市400016

出　　处：《中国动脉硬化杂志》2010年第8期589-593,共5页Chinese Journal of Arteriosclerosis

基　　金：教育部"高等学校博士学科点专项科研基金"(20095503110003)资助;重庆市卫生局中医药科研项目(2005-B-24)资助

摘　　要：目的观察电针对SD大鼠局灶脑缺血再灌注后大脑皮质胎盘生长因子及其受体Flt-1 mRNA和蛋白表达的影响,探讨电针促进局灶脑缺血再灌注后脑内血管再生的可能机制。方法采用线栓法制备SD大鼠局灶脑缺血再灌注模型。将SD大鼠随机分为正常对照组、模型组、电针组,将模型组和电针组分为局灶脑缺血2 h再灌注1、3和7天三个亚组。取双侧"合谷"穴为电针刺激穴位。采用免疫组织化学法检测胎盘生长因子及其受体Flt-1蛋白在缺血区大脑皮质的分布及其表达;逆转录聚合酶链反应检测缺血区大脑皮质胎盘生长因子mRNA和Flt-1mRNA的表达;免疫印迹法定量检测缺血区侧大脑皮质胎盘生长因子蛋白的表达。结果与正常对照组比较,模型组和电针组各时间点缺血区大脑皮质胎盘生长因子mRNA和蛋白及Flt-1 mRNA和蛋白的表达增加(P<0.05);电针组各时间点缺血区大脑皮质胎盘生长因子mRNA和蛋白及Flt-1 mRNA和蛋白的表达比模型组增加更为明显(P<0.05)。结论电针上调了局灶脑缺血再灌注大鼠缺血区大脑皮质的胎盘生长因子和Flt-1的表达,胎盘生长因子/Flt-1可能促进脑缺血后脑内血管再生。Aim To investigate the mechanism of electroacupuncture（EA） effects on promoting revascularization in SD rats brain of focal cerebral ischemia/reperfusion by discussing the expression of placental growth factor（PLGF）/Flt-1 pathway after middle cerebral artery occlusion（MCAO）. Methods The SD rats received filament occlusion of the right middle cerebral artery for 2 h.SD rats were randomly divided into control group,model group and EA group.The modle group and EA group were divided into three subgroups according to accepting reperfusion 1 d,3 d,7 d after 2 h ischemia.After 1 h of the reperfusion,EA was bilateral ＂Hegu＂point in the EA group.Immunohistochemical method was used to detect the expression of PLGF and Flt-1 protein in the cortical ischemic region.RT-PCR was used to detect the expressiom of PLGF and Flt-1 mRNA.Western blotting was employed to detect the expression of PLGF protein. Results Compared with the control group,the mRNA and protein expression of PLGF and Flt-1 in the cortical ischemic region of the model group and EA group were significantly increased（P〈0.05）.Compared with the model group,the mRNA and protein expression of PLGF and Flt-1 in the EA group were significantly increased（P〈0.05）. Conclusion EA may upregulate expression of PLGF,Flt-1 protein and PLGF,Flt-1 mRNA in the cortical ischemic region,and PLGF/Flt-1 may promote revascularization in the rats brain of focal cerebral ischemia/reperfusion.

关 键 词：电针刺激 脑缺血再灌注 胎盘生长因子 FLT-1 血管再生 

分 类 号：R363[医药卫生—病理学]