细菌启动子识别及应用研究进展  

Bacterial promoter recognition and application

作　　者：徐友强[1] 马翠卿[1] 陶飞[2] 许平[2] 

机构地区：[1]山东大学微生物技术国家重点实验室,济南250100 [2]上海交通大学微生物代谢教育部重点实验室,上海200240

出　　处：《生物工程学报》2010年第10期1393-1403,共11页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No30770064);国家高技术研究发展计划(863计划)(No2007AA10Z401);国家重点基础研究发展计划(973计划)(No2007CB707803)资助~~

摘　　要：细菌启动子是细菌中基因表达的必需调控元件,决定了细菌基因表达的强度和时机。通过启动子的插入或缺失,可以改变细菌基因的表达,实现对菌体生长发育以及代谢调控的研究。启动子也是构建各种表达系统、实现异源基因表达的基础。启动子的识别和应用研究,对于实现异源基因的可控表达、有效获得目的产物、促进生物催化和代谢工程研究具有重要的意义。以下对细菌启动子进行了简单的介绍,总结了细菌启动子的识别方法,并对细菌启动子的研究进展和具体应用进行了概述。Bacterial promoter is a kind of regulators which are needed in bacterial gene expression and decide the strength and opportunity of gene expression. By insertion or deletion of promoters, we can change bacterial gene expression in order to study the growth and metabolic regulation. Promoters are also used to construct many kinds of vectors, so as to express heterologous genes. The study of promoter recognition and application is of great importance to realize the regulation of genes, gain products effectively and promote biological catalysis and metabolic engineering. This paper reviews bacterial promoters, and the methods for recognition of bacterial promoters as well as the study and application of bacterial promoters.

关 键 词：启动子识别 诱导型启动子 启动子应用 

分 类 号：Q75[生物学—分子生物学]