超声抗原树突状细胞瘤苗抗肿瘤效应的体外研究  

Study on the anti-tumor effects of the sonicate sensitized DC vaccine in vitro

作　　者：谢艳云[1] 樊卫平[1] 李娜[1] 苑晓娟[1] 彭霞[1] 

机构地区：[1]山西医科大学微生物学与免疫学教研室,太原030001

出　　处：《肿瘤研究与临床》2010年第10期663-665,共3页Cancer Research and Clinic

基　　金：山西省科技攻关项目（20090311057-7）;山西医科大学博士启动基金（200601）;山西医科大学创新基金（01200902）;志谢感谢山西医科大学第一临床学院陈杰同学多次捐献血液

摘　　要：目的 探讨结肠癌肿瘤超声抗原致敏的树突状细胞（DC）疫苗体外对结肠癌细胞的杀伤作用.方法分离HLA-A＊0201型健康供者的外周血单个核细胞（PBMC）,重组人细胞因子rhGM-CSF、rh IL-4、rh TNF-α体外诱导DC成熟,负载SW480超声抗原后体外刺激同一供者的细胞毒性T淋巴细胞（CTL）前体,MTT法检测CTL杀伤SW480（HLA-A＊0201阳性）、K562（HLA-A＊0201阴性）的细胞毒实验.结果在效靶比20∶1、10∶1、5∶1、2.5∶1作用时,CTL对SW480的杀伤率分别为51.12%、46.67%、52.60%、40.00%;对K562的杀伤率分别为29.26%、31.98%、39.46%、31.30%,证明在各种效靶比作用时,CTL对SW480的作用均强于K562（P＜0.05）.结论肿瘤超声抗原致敏的DC瘤苗能激发具有特异杀伤功能的CTL,并且体外杀伤靶细胞具有MHC限制性.Objective To explore the cytotoxicity of the cytotoxic T lymphocyte （CTL） induced by SW480 sonicate sensitized dendritic cells （DC） on the colon cancer cell line SW480. Methods PBMC were separated from the HLA-A＊0201 donor and DC were cultured with rhGM-CSF, rhIL-4 and rhTNF-α. The same donor＇s primary CTL were stimulated by DC loaded with SW480 sonicate. The cytotoxicity of CTL on SW480 （HLA-A＊0201 positive） and K562 （HLA-A＊0201 negative） was determined by the MTT method. Results The cytotoxicity of the CTL on SW480 was stronger than that on K562 （P 〈0.05）. Conclusion The DC vaccine can stimulate specific CTL which can trigger cytotoxic activity on the target cells and this cytotoxicity is related to MHC restriction.

关 键 词：肿瘤 癌症疫苗 树突细胞 T淋巴细胞 细胞毒性 免疫 SW480 

分 类 号：R73[医药卫生—肿瘤]