不同脱细胞方法对猪主动脉瓣生物学特性的影响  

Effect of different decellularization methods on biomechanical property of porcine aortic valves

作　　者：刘春明[1] 舒礼良[1] 文冰[1] 付国伟[1] 赵文增 

机构地区：[1]郑州大学第一附属医院心血管外科,河南郑州450052

出　　处：《中国现代医学杂志》2010年第18期2733-2735,共3页China Journal of Modern Medicine

摘　　要：目的研究不同脱细胞方法对猪主动脉瓣的生物学特性的影响。方法新鲜猪主动脉瓣叶随机分为4组:新鲜瓣叶对照组(组Ⅰ)及脱细胞组(组Ⅱ、Ⅲ、Ⅳ)。脱细胞处理:组Ⅱ用0.05%胰蛋白酶;组Ⅲ用1 Tri-tonX-100+核酸酶;组Ⅳ用1 TritonX-100+核酸酶+0.05%胰蛋白酶。瓣叶分别于大鼠皮下包埋;测定生物学特性;血小板液中孵育,观察血小板黏附情况。结果组Ⅱ瓣叶纤维结构受损,极限抗张强度明显下降(P<0.05),其余2组脱细胞组性能无明显变化。组Ⅳ瓣叶皮下包埋的炎症反应较组Ⅱ、Ⅲ为轻。血小板黏附性组Ⅳ瓣叶反应较组Ⅱ、Ⅲ为轻。结论组Ⅳ脱细胞方法能更好的降低瓣叶的免疫原性并保持其生物学特性,是一种较为理想地脱细胞方法。【Objective】 To study the biomechanical properties of porcine aortic valves decellularized by different methods.【Methods】The porcine aortic valves were decellularized by 3 methods.Group Ⅰ was control group.Group Ⅱ： 0.05%Trypsin;Group Ⅲ： 1%TritonX-100＋endonuclease;Group Ⅳ： 1%TritonX-100＋endonuclease ＋ 0.05% Trypsin.Both fresh valves and decellularized valves were implanted subcutaneously in rats.Biomechanical properties of fresh valves and decellularized valves tested.【Results】The decellular matrices were intact except those in group Ⅱ,and the maximal resistance to mechanical force of matrices in group Ⅱ was obviously reduced compared to those of fresh valves（P 0.05）.The inflammation reaction of group Ⅳ were weaker than those of group Ⅱ and Ⅲ.The blood platelet adhesions of group Ⅳ were few and had no obvious deformation and pseudopodia.【Conclusion】Group Ⅳ for decellularization can completely decellularize the porcine aortic valves,keep the biome-chanical properties and reduce the immunogenicity;the scaffold made by this decellularization method may be ideal for constructing tissue-engineered heart valve.

关 键 词：猪主动脉瓣 脱细胞 生物学 心脏瓣膜 

分 类 号：R-332[医药卫生]