IGF-1在人非小细胞肺癌细胞增殖中的作用  

Effects of IGF-1 on Proliferation of Human Non-small Cell Lung Cancer Cells

作　　者：孙秀华[1] 胡海洋[1] 张洪开[1] 解智慧[1] 于爱鸣[1] 

机构地区：[1]中国医科大学生物化学与分子生物学教研室,沈阳110001

出　　处：《中国医科大学学报》2010年第9期727-729,736,共4页Journal of China Medical University

基　　金：辽宁省教育厅高校科研基金资助项目(2004D225)

摘　　要：目的探讨胰岛素样生长因子1(IGF-1)对人非小细胞肺癌(NSCLC)细胞增殖的影响及可能的分子机制。方法 MTT比色法检测0.1,1,10,100 ng/ml IGF-1对非小细胞肺癌系腺癌A549、鳞癌LK2、大细胞肺癌H460细胞增殖的影响,流式细胞术(FCM)、Western blot分析IGF-1处理前后各肺癌细胞的细胞周期变化,S期激酶相关蛋白2(SKP2)和CDC20同源蛋白1(CDH1)的表达变化。结果在非小细胞肺癌系A549、LK2、H460中,不同浓度的IGF-1均显示有促进细胞增殖的作用(P<0.05),其中1 ng/ml IGF-1作用后细胞增殖率达到峰值;与DMSO组相比,处理组S期细胞增加(P<0.01),SKP2蛋白表达增加(P<0.05),但CDH1蛋白表达却无明显变化(P>0.05)。结论非小细胞肺癌细胞中IGF-1可能是通过SKP2蛋白表达的增强,负性调控p27作用,加速细胞周期的进程。Objective To investigate the effects of insulin-like growth factor-1（IGF-1）on the cell proliferation of human non-small-cell lung cancer（NSCLC） and the possible molecular mechanism.Methods MTT assay was used to examine the effects of IGF-1 （0.1,1,10,100 ng/mL）on the cell proliferation of NSCLC cell lines（A549,LK2,H460）,Flow cytometry（FCM）and Western blot to ana-lyze the cell cycles and the protein expression of S-Phase Kinase-Associated Proteins 2（Skp2）and CDC20 homolog 1（CDH1）,respectively.Results The cell proliferation of NSCLC cell lines（A549,LK2,H460）could be promoted by the IGF-1 at different concentrations and the proliferation rate peaked when the cells were treated with 1 ng/mL IGF-1.Compared with control,the percentage of the S-phase cell population was significantly increased after the treatment of IGF-I（P 〈 0.01）and the protein expression of SKP2 also increased obviously（P 〈0.05）.However,there was no change in the CDH1 protein expression（P 〉 0.05）.Conclusion IGF-1 may accelerate the cell-cycle pro-gression of NSCLC cells by negatively modulating p27 protein via the up-regulation of SKP2 protein expression.

关 键 词：胰岛素样生长因子1 肺癌 S期激酶相关蛋白2 CDC20同源蛋白1 细胞增殖 

分 类 号：R734.202[医药卫生—肿瘤] Q78[医药卫生—临床医学]