人工加速老化对小麦种子CAT、POD、SOD活性和可溶性蛋白质含量的影响  

Effects of Accelerated Aging on CAT,POD,SOD Activities and Soluble Protein Content of Wheat Seeds

作　　者：孔治有[1] 刘叶菊[2] 覃鹏[2] 

机构地区：[1]保山学院生化系,保山678000 [2]云南农业大学农学与生物技术学院,昆明650201

出　　处：《中国粮油学报》2010年第10期24-27,共4页Journal of the Chinese Cereals and Oils Association

基　　金：云南省教育厅自然科学基金(08Y0166)

摘　　要：为探明小麦种子在种子贮藏过程中的生理生化变化的规律及合适的老化处理条件,采用高温高湿人工加速老化的方法,对5个非糯小麦和5个糯小麦品系的种子在90%相对湿度和40、45、50、55、60℃条件下分别处理0、2、4、6和8 d,结果表明:SOD、POD和CAT活性在较低温度处理下呈升高、而较高温度处理下则呈下降趋势;可溶性蛋白质含量在45℃处理下降低,但在50℃处理下升高。研究表明,小麦以90%的相对湿度和55℃的高温处理能在较短的时间内达到最佳的老化效果。Experiments were conducted to evaluate the physiological and biochemical characteristics of waxy wheat seeds during accelerated aging conditions.In present research,five waxy wheat lines,Waxy 9,Waxy 15,Waxy 4,Waxy 1,Waxy 8,and five non-waxy wheat lines,S-39,04J89,Jan-81,ⅢI42-4,and III10 were studied.The seeds were subjected to accelerated aging at 40,45,50,55,and 60 ℃,and relative humidity 90% for 0,2,4,6 and 8 days,respectively.Results： SOD,POD and CAT activities increase at low-temperature treatments,but decrease at severe treatments.Soluble protein content decreases at 45 ℃,but increases and then decreases at 50 ℃.It is revealed that RH 90% and 55 ℃ are the best accelerated aging conditions for the maximum efficiency.

关 键 词：小麦种子 人工加速老化 过氧化氢酶 过氧化物酶 超氧化物歧化酶 可溶性蛋白 

分 类 号：S37[农业科学—农产品加工]