来曲唑对子宫内膜异位症模型大鼠的作用及对生殖系统影响的研究  

Effect of letrozole on EM rat models and influence on the reproductive system

作　　者：宋淑芳[1] 宋静慧[1] 张林燕[1] 

机构地区：[1]内蒙古医学院第一附属医院妇产科,呼和浩特010059

出　　处：《中国医师杂志》2010年第10期1309-1312,共4页Journal of Chinese Physician

摘　　要：目的 探讨第三代芳香化酶抑制剂（aromatase inhibitors,AIs）来曲唑对子宫内膜异位症模型大鼠的作用及对生殖系统的影响.方法 应用自体子宫内膜移植方法建立大鼠内异症模型,将20只建模成功大鼠随机分为来曲唑组和盐水对照组,比较治疗前后大鼠子宫内膜异位症（EM）病灶体积的变化;末端转移酶介导的缺口末端标记法（TUNEL）检测细胞凋亡情况;取对侧子宫角及卵巢,称重后行HE染色观察形态学变化.结果 来曲唑组异位病灶体积缩小[（28.75±2.28）mm3 vs（108.39±9.98）mm3,P〈0.01];来曲唑组异位内膜细胞凋亡率[（5.52±2.81）%]明显高于对照组[（2.11±1.70）%,P〈0.01];来曲唑组大鼠卵巢重量明显增加[（25.25±9.89）mg/100g vs（13.10±2.70）mg/100g,P〈0.01],卵巢呈多囊样改变;来曲唑组子宫重量减轻[（41.46±15.81）mg/100gvs（94.81±18.00）mg/100g,P〈0.05],内膜呈增生抑制改变.结论 来曲唑通过促进异位内膜细胞凋亡来治疗大鼠子宫内膜异位症模型.来曲唑会引起子宫内膜异位症模型大鼠卵巢过度刺激、子宫重量减轻、子宫内膜呈增生抑制状态.Objection To study the effect of letrozole on EM rat models and influence on the reproductive system. Methods Surgically transplanted autologous uterine tissues to ectopic site beside the uterines in rats were used as animal models to study endometriosis. 20 EM model rats were random divided into letrozole-treated group and saline solution-treated control group. The change of ectopic lesion volume in each group was compared before and after treatment. Apoptotic cells were assessed by the terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick-end labeling （TUNEL） assay, and the uterian horn and ovary were weighted and observed by optical microscopy to study the change of morphology. Results The volumes of the endometriotic tissues of letrozole group reduced more than that in control group[ （ 28. 75 ± 2.28 ）mm3 vs （ 108.39 ±9. 98）mm3, P 〈0.01 ]. The apoptotic rate in letrozole group [ （5.52 ±2. 81 ）% ]was higher than control group[ （2.11 ± 1.70）%, P 〈0. 01 ]. The ovarian weights in letrozole-treated group increased significantly[ （25.25 ± 9. 89） mg/100g vs （ 13. 10 ± 2. 70 ） mg/100g, P 〈 0. 01 ], arid the ovaries showed polycyst. The uterian weights in letrozole-treated group[ （41.46 ± 15.81 ） mg/100g vs （94. 81 ±18.00） mg/100g, P 〈0. 05 ] significantly decreased, and the endometriums presented atrophy. Conclusion Letrozole treated EM by means of increasing the apoptosis of the ectopic tissues. Letrozole would give ovarian over stimulating and the uterian weighting decreased as well as endometriums atrophy.

关 键 词：芳香酶抑制剂/药理学 三唑类/药理学 子宫内膜异位症/药物疗法 泌尿生殖系统/药物作用 

分 类 号：R-332[医药卫生] R711