酒精和乙醛对神经胶质细胞膜脂质流动性的影响  

The Effects of Alcohol and Acetaldehyde on Neuroglial Membrane Lipid Fluidity in Rats

作　　者：屈卫东[1,2] 魏大鹏[1,2] 吴德生[1,2] 肖帮良 谢芳莉[1,2] 王凤英 

机构地区：[1]华西医科大学公共卫生学院环境卫生教研室 [2]华西医科大学基础医学院棉免疫教研室

出　　处：《环境与健康杂志》1999年第3期133-135,共3页Journal of Environment and Health

基　　金：四川省计生委资助

摘　　要：为研究酒精对神经胶质细胞损害的作用机制，应用荧光偏振技术探讨了酒精及其代谢产物乙醛对神经胶质细胞中星形胶质细胞和少突胶质细胞膜脂质荧光偏振度（Ｐｒ）和脂质流动度（ＬＦＵ）的影响。结果表明２ｍｍｏｌ／Ｌ酒精、乙醛并不影响星形胶质细胞、少突胶质细胞膜脂荧光偏振度和流动度，５ｍｍｏｌ／Ｌ，２０ｍｍｏｌ／Ｌ酒精和乙醛均可影响两种细胞的Ｐｒ值，导致荧光偏振度降低和细胞膜脂质流动度增高，且均与酒精和乙醛剂量相关。２０ｍｍｏｌ／Ｌ酒精对星形胶质细胞作用强于少突胶质细胞，２０ｍｍｏｌ／Ｌ乙醛对两种细胞膜脂质荧光偏振度和流动度的影响无显著性差异。酒精和乙醛导致两种神经胶质细胞膜流动性增加，致使两种细胞膜的结构发生了改变，这很可能是酒精所致神经系统发育异常的机制之一。In order to research the mechanism of damage on neurogliocytes induced by alcohol and its metabolites acetaldehyde,in this research,fluorescence polarization technique was used to explore the effects of alcohol and acetaldehyde on astrocytes and oligodendrocytes membrane lipid fluidity in rats.The results showed that 2 mmol/L alcohol and acetaldehyde had no effects on fluorescence polarization and lipid fluidity for two kinds of gliacyte,5 and 20 mmol/L alcohol and acetaldehyde could resulted in polarization(Pr) value decreased and lipid fluidity unit(LFU) increased.There were significant relationships between the Pr values and the levels of alcohol and acetaldehyde.The effects of 20 mmol/L alcohol on astrogliacytes was stronger than that of oligodendrocytes,however,the effects of 20 mmol/L acetaldehyde on two kinds of cells,presented no significant differences in Pr value and the levels of LFU.The alcoholacetaldehydeinduced increases of fluidity resulted in the variances of membrane construction in two kinds of neurogliocytes which might be one of the mechnisms for alcoholinduced developmental abnormatities in nervous system.

关 键 词：酒精 乙醛 星形胶质细胞 流动性 神经细胞 

分 类 号：R338[医药卫生—人体生理学]