恶性疟原虫地理株体外培养及var基因转录方法的建立  

Establishment of continuous cultivation of Plasmodium falciparum field isolates and transcription analysis of their var gene

作　　者：张逸龙[1] 张青锋[1] 魏桂英[1] 程倩倩[1] 潘卫庆[1] 

机构地区：[1]同济大学传染病与疫苗研究所,上海200092

出　　处：《中国热带医学》2011年第1期1-4,共4页China Tropical Medicine

基　　金：国家重点基础研究发展计划-973项目(编号:2007CB513100)

摘　　要：目的建立恶性疟原虫地理株连续体外培养方法,并采用荧光定量PCR技术分析培养虫株var基因转录类型。方法采用常规RPMI1640培养基和添加AlbumaxⅡ等成分,建立2株恶性疟原虫地理株的体外培养,并采用荧光定量PCR技术检测中晚期虫体var基因转录变化。结果成功体外连续培养2株恶性疟原虫野生株,并建立了荧光定量PCR检测var基因转录的方法,用该方法检测出该虫株var基因优势转录类别。结论建立的疟原虫体外培养方法和var基因转录检测技术可用于我国恶性疟原虫var基因致病作用的分析。Aim To establish in vitro continuous culture of plasmodium falciparum field isolates and a method for analysis of expression pattern of their vat gene. Methods Two Plasmodium falciparum field isolates were collected from malaria patients and cultured in vitro using RPMI 1640 medium supplemented with AlbumaxiI and others. The expression profile of the var gene at different development stages of Plasmodium falciparum was detected using fluorescence quantitative PCR technique to detect. Results All the field isolates were successfully recovered and continuously cultured in vitro. Quantitative PCR were successfully established for analysis of expression patterns of the vat gene and a predominant var group was detected in the two isolates using the method. Conclusion The methods established for continuous cultivation of Plasmodiumfalciparum isolates and the quantitative PCR is a useful tools for analysis of var gene transcription and study on the pathogenesis of Plasmodiumfalciparum isolates in China.

关 键 词：恶性疟原虫地理株 连续体外培养 荧光定量PCR 

分 类 号：R531.3[医药卫生—内科学]