Spondin2在结肠炎发生过程中的表达  

Spondin 2 expression is up-regulated during colitis

作　　者：黄庆文[1] 王琳[2] 周静平[2] 廉亚美[2] 

机构地区：[1]厦门大学附属中山医院湖里分院消化内科,福建省厦门市361006 [2]厦门大学附属中山医院消化内科厦门大学消化疾病研究所厦门市消化疾病中心,福建省厦门市361004

出　　处：《世界华人消化杂志》2010年第25期2690-2693,共4页World Chinese Journal of Digestology

摘　　要：目的:研究Spondin2的表达调控及其所涉及的信号通路.方法:用含3%葡聚糖硫酸钠饮用水连续喂养C57BL/6小鼠6d,诱导急性结肠炎,然后将小鼠处死,取其结肠组织提取RNA分析.通过定量RT-PCR分析Spondin2 mRNA和核因子-κBp65的表达.应用不同细胞因子和Toll样受体(toll-like receptors,TLRs)配体分别刺激细胞,RT-PCR分析Spondin2 mRNA的表达.结果:与对照组比较,在葡聚糖硫酸钠诱导小鼠结肠炎模型上,Spondin2的mRNA表达上调4.5±0.5倍.TLR-9配体,CpG-ODN刺激野生型RAW264.7细胞后,Spondin2表达上调4.3±0.4倍.结论:在结肠炎发生过程中Spondin2的mRNA表达上调,并可能通过了TLR-9介导的信号通路.AIM：To investigate the regulation of Spondin 2 expression during colitis and to analyze potential signaling pathways involved.METHODS：C57BL/6 mice were treated with 3% dextran sulfate sodium for 6 d to induce acute colitis.The colon tissue specimens were then harvested for RNA isolation.The mRNA expression of Spondin 2 and nuclear factor-κB p65 was analyzed by quantitative RT-PCR.After RAW 264.7,CMT93 and HEK293 cells were stimulated with different cytokines and Toll-like receptor ligands,the mRNA expression of Spondin 2 was analyzed.RESULTS：The mRNA expression of Spondin 2 was up-regulated by 4.5 ± 0.5 folds in the colon of mice with acute colitis compared with control mice.CpG oligodeoxynucleotide（CpG ODN）,a Toll-like receptor 9（TLR-9） ligand,induced a 4.3 ± 0.4-fold up-regulation of Spondin2 expression in RAW 264.7 cells.CONCLUSION：Spondin 2 expression is upregulated during colitis perhaps in a TLR-9dependent manner.

关 键 词：Spondin2 结肠炎 TOLL样受体9 

分 类 号：R574.6[医药卫生—消化系统]