CTGF在肝细胞癌中的表达及作用  

Expression and roles of connective tissue growth factor in human primary hepatocellular carcinoma

作　　者：张瑞娟[1] 邹亚斌[1] 高润平[1] 张秀军[1] 朱杰[1] 

机构地区：[1]吉林大学第一医院肝胆胰内科,吉林省长春市130021

出　　处：《世界华人消化杂志》2010年第26期2775-2779,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30872236~~

摘　　要：目的:探讨在细胞水平上结缔组织生长因子(connective tissue growth factor,CTGF)在人肝细胞癌(hepatocellular carcinoma,HCC)生长和转移中发挥的作用.方法:采用原位杂交技术检测36例HCC手术切除标本和6例正常肝组织中CTGF mRNA,计算机图像分析系统测定癌灶及癌周基质CTGF mRNA阳性细胞的IA值,试剂盒测定CTGF刺激肝癌细胞株HepG2细胞的迁移、侵袭力,流式细胞仪测定HepG2细胞周期.结果:原位杂交结果显示CTGF mRNA主要在癌周结缔组织及动、静脉壁中表达.在细胞水平研究结果显示:重组CTGF可明显提高HepG2细胞的迁移和侵袭力(均P<0.01);CTGF可使HepG2细胞周期中S期细胞数明显增加(10.94±0.23vs23.85±2.35,P<0.01).结论:CTGF在HCC生长和转移中发挥重要的作用,其有可能成为一个新的治疗靶点.AIM： To detect the expression of connective tissue growth factor （CTGF/CCN2） in human hepatocellular carcinoma （HCC） and to elucidate its roles in the development of HCC in vitro. METHODS： Liver samples from 36 HCC patients, who underwent hepatic resection, and 6 normal individuals were used to detect CTGF mRNA expression by in situ hybridization. Computer imaging analysis was performed to measure the integrated absorbance （IA） of CTGF mRNA-positive cells in carcinoma and surrounding stroma. The impact of CTGF on HepG2 cell migration and invasion was determined using commercial kits. Cell cycle analysis was performed by flow cytometry.RESULTS： In situ hybridization analysis showed a significance increase in CTGF mRNA expression that was mainly detected in connective tissue and blood vessels around carcinoma foci. Incubation of HepG2 cells with 100 ng/mL CTGF significantly promoted cell migration and invasion （both P 0.01）. Cell cycle analysis showed that addition of 100 ng/mL CTGF to HepG2 cell culture medium resulted in more cells progressing into Sphase （10.94 ± 0.23 vs 23.85 ± 2.35, P 0.01）. CONCLUSION： CTGF may play important roles in the development of HCC and is a potential novel therapeutic target for HCC.

关 键 词：结缔组织生长因子 肝细胞癌 肝癌细胞株 迁移 侵袭 细胞周期 

分 类 号：R735.7[医药卫生—肿瘤]