高效液相色谱法测定凤仙花植株不同部位槲皮素、山奈酚含量  

HPLC Determination of Contents of Quercetin and Kaempferol in Different Positions of Impatiens Balsamina Plant

作　　者：李猛[1] 艾惜[1] 孙建厅[1] 王一飞[1] 田婧[1] 边清泉[1] 

机构地区：[1]绵阳师范学院化学与化学工程学院,绵阳621000

出　　处：《理化检验（化学分册）》2010年第10期1160-1162,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：绵阳市科技局自然科学研究项目(10Y002-11);绵阳师范学院科学研究项目(fx08-09-1;fx08-09-2)

摘　　要：建立了高效液相色谱法测定风仙花不同部位槲皮素和山萘酚含量的方法,并对含量变化进行比较。用DiamonsilTMC18（4.6 mm×250 mm,5μm）为色谱柱,甲醇与乙酸（0.5＋99.5）以体积比65比35混合溶液为流动相,流量为0.9 mL.min-1,检测波长为365 nm。测得槲皮素回收率为101.1%,相对标准偏差（n=6）为1.5%,线性范围为0.84~6.72 mg.L-1之间;山奈酚回收率为96.0%,相对标准偏差（n=6）为2.6%,线性范围在2.56~20.48 mg.L-1之间。经比较试样的分析结果发现,槲皮素含量在未开花叶中最高（0.112 mg.g-1）,而山奈酚含量在花中最高（0.972 mg.g-1）。Contents of quercetin and kaempferol,as the active medicative components of Impatiens balsamina in different positions of its plant were determined by HPLC and a comparative study was made.The DiamonisilTM C18（4.6 mm×250 mm,5 μm） column was used stationary phase,and a mixture of methanol and dil.acetic acid（0.5＋99.5） mixed in the ratio of 65 to 35（pH 3.5） was used a mobile phase with a flow-rate of 0.9 mL·min-1.UV-detection was made at the wavelength of 365 nm.Linear relationship between values of peak area and concentration was obtained in the range of 0.84-6.72 mg·L-1 for quercetin,and in the range of 2.56-20.48 mg·L-1 for kaempferol.Tests of recovery were made by standard addition method,and values of average recovery found were 101.1% and 96.0% with RSD′s（n=5） of 1.5% and 2.6% respectively.It was shown that highest content of quercetin（0.112 mg·g-1） was found in the leaves before blossoming and highest content of kaempferol（0.972 mg·g-1） was found in the flower.

关 键 词：高效液相色谱法 槲皮素 山奈酚 凤仙花 

分 类 号：O652.63[理学—分析化学]