超声波-酶法提取菊花中总黄酮工艺优化  

Extraction study of total flavonoids from chrysanthemum by ultrasonic wave-cellulase

作　　者：刘小锐[1] 梅林[2] 

机构地区：[1]西南大学化学化工学院,重庆400715 [2]后勤工程学院应管系,重庆400016

出　　处：《激光杂志》2010年第5期95-96,共2页Laser Journal

摘　　要：目的:优化超声波-酶法提取菊花中总黄酮的工艺。方法:考察不同的影响因素,采用L9(34)正交设计优化提取工艺。以芦丁为对照品,以紫外分光光度计分析其提取率。结果:乙醇浓度具有显著性影响,超声波-酶法提取杭菊中总黄酮最佳条件为乙醇浓度为70%,酶添加量为0.4%,在55℃下酶解超声60+20min,其总黄酮得率为6.40%。结论:本工艺简单可行、高效快速,得率比酶解法提高了19.9%。Objective：To obtain the optimum operation conditions for extraction of total flavonoids from chrysanthemum with Ultrasonic Wave-Cellulase.Methods：Investigated the influencing factors,with L9（3^4） orthogonal design optimized extraction process.Results：The alcohol concentration is the best effect factor and it has significant impact.The results show that optimum conditions are that alcohol concentration was 70%,the amount of cellulose was 0.4%,enzymolysis temperature was 55？欥nzymolysis-ultrasonic time was 60 and 20min.Flavones yield was 6.40%.Conclusion：The technological process is simple,steady and effective.Comparing with extraction ratio of enzymatic method,it increases 19.85%.

关 键 词：杭菊花 总黄酮 纤维素酶 提取 正交设计 

分 类 号：R477[医药卫生—护理学]