鹿蹄橐吾水溶性多糖的分离纯化和体外清除自由基活性研究  

Study on the Purification and Scavenging Free Radical Activity of Water Soluble Polysaccharide from Ligularia hodgsonii

作　　者：刘春兰[1] 李阳[1] 杜宁[1] 黄潇[1] 王文蜀[1] 

机构地区：[1]中央民族大学生命与环境科学学院,北京100081

出　　处：《中药材》2010年第9期1414-1416,共3页Journal of Chinese Medicinal Materials

基　　金："111创新引智计划"工程项目(B08044);中央民族大学"985"工程重点科研项目;中央民族大学2009-2010年度自主科研计划项目(自由探索类)

摘　　要：目的:为合理利用鹿蹄橐吾提供一定依据,对鹿蹄橐吾进行多糖提取分离纯化。方法:鹿蹄橐吾经热水浸提,乙醇沉淀得粗多糖LW。此粗多糖经分离纯化得纯化多糖LW21。LW21经检测为纯多糖。用荧光法对鹿蹄橐吾多糖LW,LW21进行清除自由基活性的研究。结果:LW、LW21对羟自由基及超氧阴离子自由基具有明显的清除效果,且LW21对上述两种自由基的清除效果优于粗多糖LW。结论:从清除自由基活性实验可以证明鹿蹄橐吾水溶性多糖可作为抗衰老保健或药物在临床上使用,具有进一步开发生产的价值。鹿蹄橐吾多糖LW21是一新结构多糖,为首次从鹿蹄橐吾中分离得到。Objective：The study was to provided the reference for futher development and utilization of Ligularia hodgsonii.Methods：The crude polysaccharide LW was extracted from Lugularia hodgsonii with hot water,and prectated by ethanol.The crude polysaccharide has been fractionted and purified,so LW21was got.Its homogeneity was confrimed by chromatography on Sepharose CL-4B,paper chromatography and cellulous actate electrophoresis.The scavenging effets of the polysacehardes on ·OH and O-2· were determined by fluorescence method.Results：Antioxidative experiment shows that LW and LW21 were effective in scavenging superoxide radical and hydroxyl radical.The scavenging capacity for superoxide radical and hydroxyl radical of LW21 had higher than that of LW.Conclusion：The polysaceharide from Ligularia hodgsonii had stronger antioxidant actioxdant activities,and their antioxidant activities had certain dose-relationship.

关 键 词：鹿蹄橐吾 多糖 纯化 自由基 

分 类 号：R284.1[医药卫生—中药学] R285.5[医药卫生—中医学]