中药复方凉茶提取物体外抗HIV活性研究  

Anti-HIV Activities of Extracts from Chinese Medicinal Fromula Liangcha

作　　者：刘武青[1,2] 李磊珂[3,4] 王睿睿[1] 杨柳萌[1] 彭涛[3,4] 郑永唐[1] 

机构地区：[1]中国科学院昆明动物研究所/云南省动物模型与人类疾病机理重点实验室,云南昆明650223 [2]中国科学院研究生院,北京100039 [3]中国科学院广州生物医药与健康研究院,广东广州510663 [4]中国科学技术大学生命科学学院,安徽合肥230027

出　　处：《中药材》2010年第9期1433-1438,共6页Journal of Chinese Medicinal Materials

基　　金：国家重点基础研究发展计划(973)(2009CB522306);国家科技重大专项“十一五”计划(2008ZX10005-005,2008ZX10001-015,2009ZX09501-029);中国科学院知识创新工程重要方向(KSCX1-YW-R-24);云南省科技计划(2007BC006)

摘　　要：目的:筛选和评价中药复方凉茶提取物体外对HIV的抑制活性,并初步探讨其作用机制。方法:通过观察病毒致细胞病变和HIV-1 p24抗原表达抑制性实验,采用多株HIV病毒株(实验株、临床分离株、耐药株)对中药复方凉茶水提醇沉物体外抗HIV活性进行评价;通过融合阻断实验、HIV-1慢性感染细胞病毒复制抑制实验和重组HIV-1 RT酶活性抑制实验,初步探讨其抑制HIV复制作用机理。结果:中药复方凉茶提取物对不同HIV-1病毒株均有很好的抑制作用,其EC50值介于12.74～116.87μg/mL,对HIV-2毒株也有一定的抑制活性。中药复方凉茶提取物显示出低细胞毒性,对不同来源的细胞系的CC50值介于564.79～1699.22μg/mL。中药复方凉茶提取物能显著抑制重组HIV-1 RT酶活性,在提取物浓度5.3μg/mL时的抑制率(50%,对正常细胞与慢性感染细胞融合也有一定的抑制作用,EC50为101.94μg/mL。结论:中药复方凉茶提取物体外具有较好的抑制HIV-1复制活性,其作用机制可能是抑制HIV-1逆转录酶活性和病毒进入细胞。Objective：To screen and evaluate the anti-HIV activities of extracts from Chinese medicinal formula Liangcha.Methods：The anti-HIV-1 activities of extracts from Chinese medicinal formula Liangcha were evaluated by cytotoxicity assay,syncytium reduction assay,protection for HIV-1 induced lytic assay,and ELISA assay for HIV-1 p24 antigen expression.The primary mechanisms were investigated by fusion inhibition assay,inhibition of viral replication in HIV-1 chronically infected H9 cell and inhibition assay of HIV-1 RT activity.Results：The extracts from Chinese medicinal formula Liangcha exhibited potent and broad-spectrum anti-HIV-1 activity on different HIV-1 strains with EC50 range 12.74～116.87 μg/mL,but weakly inhibited HIV-2 replication.Meantime,it was not significantly cytotoxic in several T lymphocytes cell lines with CC50 range 564.79～1699.22 μg/mL.The activity of recombinant HIV-1 reverse transcriptase was inhibited by extracts from Chinese medicinal formula Liangcha,inhibition rate more than 50% at the concentration of 5.3 μg/mL.It also weakly inhibited the cell-to-cell fusion in co-culture with EC50 of 101.94 μg/mL.Conclusion：The extracts from Chinese medicinal formula Liangcha exhibited potent and broad-spectrum anti-HIV-1 activity on different HIV-1 strains in vitro.Its anti-HIV-1 mechanism might be inhibiting HIV-1 reverse transcriptase and cell entry.

关 键 词：凉茶 艾滋病 抗HIV 火炭母 棉茵陈 木棉花 

分 类 号：R285.5[医药卫生—中药学]