九眼独活类黄酮的提取和抗氧化活性研究  

Extraction and Antioxidant Activity of Flavonoids from Aralia cordata

作　　者：刘向鸿[1] 侯大斌[1] 赵纳[1] 王博[1] 

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010

出　　处：《中药材》2010年第9期1484-1487,共4页Journal of Chinese Medicinal Materials

基　　金：四川省农作物育种攻关课题(2006YZGG12-22);绵阳市重点科技项目(06S041-05)

摘　　要：采用单因素分析结合正交试验的方法,研究了九眼独活类黄酮超声辅助提取的工艺条件,利用清除DP-PH自由基的能力和还原力作为指标测定了九眼独活类黄酮的抗氧化活性。结果表明:九眼独活类黄酮提取的优化工艺条件为乙醇体积分数30%,提取时间60 min,固液比1∶75,提取温度80℃,此时类黄酮的提取率为1.35%,九眼独活类黄酮对DPPH自由基有较好的清除效果,呈明显的剂量-效应关系,清除率达到78.88%,同时其还原力与浓度也呈现良好的线性关系(R2=0.9855)。In this study,microwave-assisted extraction techniques of flavonoids fom Aralia cordata were studied by the single factor analysis and orthogonal test,then the property antioxidant activities of flavonoids extracts were evaluated by two antioxidant assays,for 1,1-diphenyl-2-picrylhydrazyl（DPPH） radicals scavenging and reducing power.The results showed that the optimum conditions were as follows：twice extracting Aralia cordata with 75 times volume（V/W） of 30% ethanol at 140W of microwave for 60 min at 80℃.Under these conditions,the extraction yield was 1.35%.The flavonoids extracts could significantly scavenge DPPH and superoxide anion free radicals in dose-dependent manner,meanwhile a linear correlation between its concentration and reducing power was also observed（R2=0.9855）.

关 键 词：九眼独活 类黄酮 提取 抗氧化 

分 类 号：R931.71[医药卫生—生药学]