雌激素对谷氨酸诱导大鼠脑皮质细胞凋亡的影响  

Effect of estrogens on Glutamate-induced apoptosis and expression of apoptosis gene of bcl-2,bax in rat cortical neurons

作　　者：杨卫民[1] 周农[1] 施雪英[1] 

机构地区：[1]安徽医科大学第一附属医院,合肥230022

出　　处：《安徽卫生职业技术学院学报》2010年第5期70-71,共2页Journal of Anhui Health Vocational & Technical College

基　　金：安徽省教育厅自然科学基金资助项目(编号:2002jk163)

摘　　要：目的:观察雌激素对谷氨酸诱导离体皮层神经元凋亡的作用,探讨雌激素的神经保护作用机制。方法:原代培养大鼠大脑皮质细胞,分为正常对照组、谷氨酸组和谷氨酸+雌激素组,采用TUNEL和免疫细胞化学检测等方法,分析雌激素对谷氨酸诱导神经元凋亡的作用。结果:谷氨酸可明显诱导神经元凋亡,凋亡指数为34.6%;雌激素能明显拮抗谷氨酸的凋亡毒性作用,凋亡指数下降为18.4%(与谷氨酸组相比P<0.05);免疫组化结果显示,雌激素组bcl-2表达阳性细胞明显增加,bax蛋白表达受到抑制。结论:雌激素具有神经保护作用,作用机制可能通过影响凋亡相关基因的表达来实现。Objective：To observe the neuroprotection role and its mechanism of estrogens on apoptosis induced by Glutamate in primary rat cortical neuronal culture.Methods：Using primary rat cortical neuronal culture,TUNEL and immunocytochemistry were carried to assess the effect of estrogens on Glutamate-induced neuron apoptosis and the expression of bcl-2,bax.Results：Estrogens protects against Glutamate-induced neuronal apoptosis,higher expression of bcl-2 and lower expression of bax were also found.Conclusions：Estrogens may play protective role on primary rat cortical cultures.Rrgulation of apoptosis-linked gene expression is involved in its mechanisms.

关 键 词：雌激素 细胞凋亡 BCL-2 BAX 

分 类 号：R-33[医药卫生]