肺微血管内皮细胞的原代培养  

Primary culture of pulmonary microvascular endothelial cells in rats

作　　者：时全星[1] 孙新[2] 李娟[1] 郭海涛[1] 殷玥[1] 毕辉[1] 胡玉珍[1] 王跃民[1] 裴建明[1] 

机构地区：[1]第四军医大学基础部生理学教研室,陕西西安710032 [2]西京医院儿科,陕西西安710032

出　　处：《现代生物医学进展》2010年第19期3609-3612,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金资助项目(NO:30900535;30971060;30700345;30770802)

摘　　要：目的:建立简单高效获取大鼠肺微血管内皮细胞(pulmonary microvascular endothelial cells,PMVECs)的培养方法。方法:(1)组织块贴壁法培养大鼠PMVECs;(2)光镜下观察细胞的形态;(3)扫描电镜和透射电镜分别观察细胞表面及内部的结构特征;(4)免疫荧光法鉴定PMVECs。结果:(1)获得的PMVECs长满后呈典型的铺路石或鹅卵石状;(2)扫描电镜下可见内皮细胞表面存在微绒毛等特殊结构;(3)透射电镜观察可见细胞浆内存在韦伯潘力氏小体(WebelPaladebody,WPB);(4)免疫组化结果显示细胞浆中有Ⅷ因子相关抗原存在。结论:改良的组织块法培养肺微血管内皮细胞是一种简单、快捷,有效的方法。Objective： To establish a simple and reproductable method to culture the rat pulmonary microvascular endothelial cells. Methods： （1）. Tissue block pasted culture method was used to isolate and culture PMVECs; （2）. The morphous of cultured cells were detected by microscopy; （3）. Structural feature of cell surface and interior were observed by scanning electron microscope and transmission electron microscope respectively; （4）. PMVECs were identified by immunofluorescence histochemistry. Results： （1）. The morphous of cultured primary PMVECs had a typical cobblestone-like and pavingstone shape; （2）. Lots of microvilli appeared on the surface of the PMVECs under scanning electron microscope; （3）. Webel Palade body appeared in the cytoplasm of PMVECs; （4）. Immunofluorescence histochemistry staining of factor Ⅷ-associated antigen was positive. Conclusion： Tissue block pasted is a simple, rapid and effective method to culture pulmonary microvascular endothelial cells.

关 键 词：肺 微血管内皮细胞 大鼠 

分 类 号：R332.2[医药卫生—人体生理学] Q95-3[医药卫生—基础医学]