重型乙型肝炎病毒C基因启动子变异  

Hepatitis B virus precore/core promoter mutantion in fulminant hepatitis

作　　者：郭亚兵[1,2] 戴炜[1,2] 卢桥生[1,2] 侯金林 冯筱榕[1,2] 骆抗先 

机构地区：[1]第一军医大学南方医院 [2]深圳市东湖医院

出　　处：《中华传染病杂志》1999年第2期108-110,共3页Chinese Journal of Infectious Diseases

基　　金：国家自然科学九五重点课题基金

摘　　要：目的了解我国重型肝炎Ｃ基因调节序列变异特点。方法克隆及聚合酶链反应（ＰＣＲ）产物直接测序方法对，检测７例重型乙型肝炎患者乙型肝炎病毒（ＨＢＶ）Ｃ基因调节序列变异。结果４例存在Ｔ１７６２Ａ１７６４变异；但在Ｔ１７６２Ａ１７６４以外位点也存有诸多变异，其中１例在ｎｔ１７７６－１７７７之间有１１ｂｐ的插入变异。结论重型肝炎ＨＢＶＣ基因启动子区存在较多的变异位点，推测这些变异对Ｃ启动子的调节活性影响可能是综合性的。Objective To characterize mutations in core regulatory sequences of hepatitis B virus in Chinese patients with fulminant hepatitis.Methods Seven patients with fulminant hepatitis were investigated for mutations in core gene regulatory sequences of hepatitis B virus using PCR products and cloning sequencing methods.Results Four in the seven patients had T 1762 A 1764 mutation.Furthermore,there were a lot of variants at other sites in this promoter region,induding one case with a 11bp insert between nt 1776 1777.Conclusion It indicated that there were quite a few mutation sites in the region of core promoter,in the patients with fulmiannt hepatitis and those mutations may effect the activity of core promoter synthetically.

关 键 词：乙型肝炎病毒 启动子 变异 C基因 

分 类 号：R512.62[医药卫生—内科学]