Degradation of a fullerene end-capped polycaprolactone by lipase AK  

作　　者：PENG QiYun KANG Feng LI Juan YANG XinLin 

机构地区：[1]School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China

出　　处：《Chinese Science Bulletin》2010年第29期3279-3282,共4页

基　　金：supported by the National Natural Science Foundation of China (20672012);Beijing Municipal Natural Science Foundation (20822020)

摘　　要：Using titrimetric method,enzymatic behavior of lipase AK against fullerene end-capped polycaprolactone(C60-PCL) was studied for the first time,compared with polycaprolactone(PCL).The results showed that degradation of both C60-PCL and PCL by lipase followed Mechaelis-Menten equation,with the degradation parameters of PCL and C60-PCL were 0.75 mg mL-1 and 0.16 mg mL-1 for Km,and 0.90 mL h-1 and 0.43 mL h-1 for Vmax,respectively.C60-PCL had a slower degradation rate than PCL under the same conditions.These data indicated that the introduction of fullerene enabled PCL hard to be degraded by lipase.Using titrimetric method, enzymatic behavior of lipase AK against fullerene end-capped polycaprolactone （C60-PCL） was studied for the first time, compared with polycaprolactone （PCL）. The results showed that degradation of both C60-PCL and PCL by lipase followed Mechaelis-Menten equation, with the degradation parameters of PCL and C60-PCL were 0.75 mg mL1 and 0.16 mg mL 1 for Km, and 0.90 mL h-1 and 0.43 mL h- 1 for Vmax, respectively. C60-PCL had a slower degradation rate than PCL under the same conditions. These data indicated that the introduction of fullerene enabled PCL hard to be degraded by lipase.

关 键 词：聚己内酯 脂肪酶 富勒烯 降解酶 封端 PCL C60 米氏方程 

分 类 号：O633.14[理学—高分子化学] TQ925.6[理学—化学]