高尔基体α-甘露糖苷酶Ⅱ在胃癌中的表达及其与E-cadherin、Galectin-3的关系  

Significance of golgi α-mannosidase Ⅱ,E-Cadherin,Galectin-3 expression and their correlation in human gastric carcinoma

作　　者：杨雅莹[1] 李艳青[1] 易永芬[1] 何婷婷[1] 肖钟[1] 

机构地区：[1]重庆医科大学基础学院病理学教研室、分子医学与肿瘤研究中心,重庆400016

出　　处：《重庆医科大学学报》2010年第9期1313-1317,共5页Journal of Chongqing Medical University

基　　金：国家自然科学基金(编号:30672431);教育部博士点基金(编号:20060631006)

摘　　要：目的:探讨胃癌高尔基体α-甘露糖苷酶Ⅱ(Golgiα-mannosidaseⅡ,GMⅡ)、E-cadherin、Galectin-3的表达及其与胃癌发生发展的关系。方法:应用免疫组化SP法检测65例胃癌,20例正常胃黏膜组织中GMⅡ、E-cadherin、Galectin-3的表达情况。结果:65例胃癌中GMⅡ、E-cadherin、Galectin-3的阳性表达率分别为85%(55/65)、40%(26/65)、74%(48/65)。20例胃正常切缘中GMⅡ、E-cadherin、Galectin-3的阳性表达率分别为50%(10/20)、100%(20/20)、40%(8/20)。GMⅡ、Galectin-3在浸润程度深及有淋巴结转移的胃癌组织中表达明显高于浸润程度浅及无淋巴结转移的胃癌组织(P<0.05)。而E-cadherin则在分化程度低,浸润程度深及有淋巴结转移的胃癌组织中表达明显低于分化程度高,浸润程度浅及无淋巴结转移的胃癌组织(P<0.05)。统计学分析GMⅡ与E-cadherin之间呈负相关(P<0.05),与Galectin-3无相关性(P>0.05)。结论:GMⅡ、Galectin-3在胃癌中高表达,且与癌组织的浸润和转移明显相关。同时GMⅡ与E-cadherin呈负相关,提示GMⅡ可能通过下调E-cadherin的表达来促进胃癌的发展。Objective：To investigate the expression of GMⅡ,E-cadherin,and Galectin-3,and their roles in tumorigenesis and progression of gastric cancer.Methods：Immunohistochemistry SP method was used to determine expression of GMⅡ,E-cadherin,and Galectin-3 in gastric carcinoma（n=65） and normal gastric mucosa（n=20）.Results：The positive rates of GMⅡ,E-cadherin,and Galectin-3 were respectively 85%（55/65）,40%（26/65）,and 74%（48/65） in gastric cancer ;their were respectively 50%（10/20）,100%（20/20）,and 40%（8/20） in normal gastric mucosa.The expressions of GMⅡ,E-cadherin,and Galectin-3 were significantly different between tissues from carcinoma and those from normal mucosa（P0.05）.The expressions of GMⅡand E-cadherin were significantly related to poor differentiation,deep invasion and lymph node metastasis.GMⅡ expression had a negative correlation with E-cadherin（P0.05）,but no correlation with galectin-3（P0.05）.The expression of galectin-3 was markedly related to the depth of invasion,lymph node metastasis,but not to tumor differentiation.Conclusion：Up-regulated expression of GMⅡ,Galectin-3 play important roles in the invasion and metastasis of gastric carcinoma.There is a significant negative correlation between GMⅡ and E-cadherin,suggesting that GMⅡ may promote the development of gastric carcinoma through down-regulating expression of E-cadherin.

关 键 词：胃癌高尔基体α-甘露糖苷酶Ⅱ E-CADHERIN GALECTIN-3 胃癌 免疫组化 

分 类 号：R361[医药卫生—病理学]