CML细胞eIF4E和Trib2基因mRNA表达分析  

mRNA expression of eIF4E and Trib2 gene in CML patients and K562 cells

作　　者：黄莲芬 冯文莉[3] 万根平[1,2] 罗红伟 

机构地区：[1]广州市妇女儿童医疗中心 [2]广州市儿童医院检验科,广州510120 [3]重庆医科大学医学检验系临床检验诊断学省部共建教育部重点实验室,重庆400016

出　　处：《重庆医科大学学报》2010年第9期1358-1360,共3页Journal of Chongqing Medical University

摘　　要：目的:为了探索慢性粒细胞白血病(Chronic myelogenous leukemia,CML)急变机理,寻求新的治疗靶标,我们对获得的9例CML病人骨髓标本以及CML急性红系变细胞株中K562细胞株中eIF4E基因和Trib2基因的表达水平进行初步检测,为进一步的研究奠定基础。方法:分离骨髓单个核细胞,用Trizol裂解提取RNA,RT-PCR检测eIF4E基因和trib2基因mRNA的表达水平。结果:初步分析显示部分CML病人eIF4E基因mRNA表达水平很高,为CML疾病进展机制进一步研究奠定了基础。结论:部分CML病人骨髓标本中eIF4E和TRIB2基因mRNA表达水平较高,eIF4E和trib2有可能成为CML病人新的治疗靶点。Objective：To explore the mechanism of CML-BC and the corresponding new therapeutic targets by investigating the mRNA expression of eIF4E and trib2 gene in 9 CML patients and K562 cells.Methods：After isolation of BM mononuclear cells and the following RNA extraction,RT-PCR was taken to show the mRNA expression of eIF4E and trib2 gene in 9 CML patients and K562 cells.Results：Electrophoresis analysis showed that both mRNA expression of eIF4E and trib2 gene were high in some CML patients especially in CML-BC and K562 cells,which laid foundation for further investigation on CML development.Conclusion：Both mRNA expression of eIF4E and trib2 gene were high in some CML patients and K562 cells,they may be the possible therapeutic targets for CML patients.

关 键 词：慢性粒细胞白血病 真核细胞翻译起始因子4E Trib2 

分 类 号：R730.231[医药卫生—肿瘤] R733.7[医药卫生—临床医学]