小鼠胚胎腭板发育过程中麦胚凝集素、蓖麻凝集素和鸡冠珊瑚树凝集素受体的表达  

Effects of lectin receptors of wheat germ agglutinin, ricinus communis agglutinin, erythrina cristagaili lectin in the development of miouse palate

作　　者：段淑红[1,2] 刘凯[2] 尹海燕[3] 刘丰韬[1] 窦珊姗[2] 

机构地区：[1]山东大学第二医院,济南250012 [2]山东大学医学院组织学与胚胎学教研室,济南250012 [3]蚌埠医学院组织学与胚胎学教研室,蚌埠233000

出　　处：《解剖学杂志》2010年第5期606-609,共4页Chinese Journal of Anatomy

基　　金：山东省自然科学基金（Y99COI）

摘　　要：方法：以生物素标记的凝集素WGA、 RCA和ECL为探针,应用亲和组织化学显色方法检测上述3种凝集素受体在各组小鼠胚胎腭板上皮及其间充质的分布状况和变化规律.结果：在小鼠胚胎腭发育过程中3种凝集素受体在腭板上皮中的表达水平均高于在间充质中的表达水平.WGA受体在腭板上皮中的表达峰值出现于d1322-d148,即腭板上抬和水平生长期;而RCA和ECL受体的表达峰值出现于d158-d1522,即腭板融合期.结论：3种凝集素受体均可能不同程度地参与腭板正常发育的调节,WGA受体可能主要作用于腭板上抬和水平增长期,而RCA和ECL受体则可能主要作用于腭板融合期.Objective： To explore the relationship between the development of mouse embryonic palate and expression patterns of receptors of lectin wheat germ agglutinin （WGA）, ricinus communis agglutinin （RCA） and erythrina cristagalli lectin （ECL）. Methods： Biotins labeled WGA, RCA and ECL were used as probes to investigate the distribution and dynamic changes of their receptors in the epithelium of embryonic palatal shelves and the mesenchyme by affinity histochemistry. Re- sults： During the development of the murine palatine shelves, the expression levels of 3 kinds of lectin receptors in palatine epithelium were significantly higher than those in the mesenchyme. The expression peak of the WGA receptor in palatine epithelium appeared at d132Z-d148 , the elevating and horizontal-growth stages, but that of RCA and ECL appeared at d158- d1522 , the fusion stage. Conclusion： The 3 kinds of lectin receptors may take part in the regulation of normal development of murine palate, the WGA receptor may regulate the elevating and horizontal-growth of the palatine shelves, and the RCA and ECL may regulate the fusion of the palatine shelves.

关 键 词：凝集素受体 腭板 发育 小鼠 

分 类 号：R735.2[医药卫生—肿瘤]