重组人改构体酸性成纤维细胞生长因子对帕金森病大鼠中脑腹侧被盖区酪氨酸羟化酶神经元的保护  

Modified recombinant human aFGF protects tyrosine hydroxylase neurons in ventral tegmental area of Parkinson＇s disease rat model

作　　者：肖春苟[1] 沈伟哉[2] 郭国庆[1] 李校堃[3] 

机构地区：[1]韶关学院医学院解剖学教研室,韶关512026 [2]暨南大学医学院解剖学教研室,广州510630 [3]温州医学院生物与天然药物研究院,温州325027

出　　处：《解剖学杂志》2010年第5期655-659,共5页Chinese Journal of Anatomy

基　　金：863国家基金（2001AA215131）

摘　　要：目的：探讨重组人改构体酸性成纤维细胞生长因子（Mrh-aFGF）对帕金森病（PD）大鼠旋转行为和中脑腹侧被盖区酪氨酸羟化酶（TH）免疫阳性神经元的影响.方法：6-羟基多巴胺（6-OHDA）分别注入黑质和腹侧被盖区后建立PD大鼠模型,侧脑室内注射Mrh-aFGF,用阿扑吗啡诱导旋转行为,免疫组织化学显色观察TH免疫阳性神经元和纤维,并进行定量分析.结果：PD组术后旋转启动时间缩短,持续时间延长,速度加快;Mrh-aFGF处理组旋转行为有改善.各组大鼠组内健侧和损毁侧阳性神经元比较,对照组损毁侧无明显改变;PD组、NS处理组和Mrh-aFGF处理组损毁侧阳性神经元与健侧比较均减少.Mrh-aFGF处理组损毁侧中脑腹侧被盖区阳性神经元数量较PD组及对照组增加.结论：Mrh-aFGF能减少PD大鼠中脑腹侧被盖区TH免疫阳性神经元的丢失,并改善其旋转行为.Objective： To observe the changes of rotation behavior and tyrosine hydroxylase immunopositive neurons and in- vestigate how Mrh-aFGF affects them in the ventral tegmental area of Parkinson＇s disease （PD） rats. Methods： After building a rat model of PD by injecting 6-OHDA into substantia nigra and ventral tegmental area, Mrh-aFGF was used to intervene rats by lateral ventricle injection to observe behavior of rats induced by apomorphine and tyrosine hydroxylase immunopositive neurons in the ventral tegmental area of rats and then quantitatively analyzed the change of tyrosine hydroxylase immunopositive neurons. Results： Rotation behavior was found in PD rats, actuation time was shorted, time length was prolonged, and average velocity of rotation was accelerated. Compared with the PD group, rotation behavior of rats treated with Mrh-aFGF was improved. The immunopositve neurons of tyrosine hydroxylase were decreased significantly in iniured side in PD, NS and Mrh-aFGF groups. After treated with Mrh-aFGF, the structure of ventral tegmental area was significantly improved and the number of tyrosine hydroxylase neurons was increased. Conclusion.. Mrh-aFGF could protect immunopositive neurons of ventral tegmental area from loss and improve the rotation behavior of PD rats.

关 键 词：帕金森病 重组人改构体酸性成纤维细胞生长因子 酪氨酸羟化酶 中脑腹侧被盖区 免疫组织化学 大鼠 

分 类 号：R737.31[医药卫生—肿瘤]