地塞米松和维生素B_(12)对小鼠胚胎腭突发育早期成纤维生长因子10及其2b受体信号的影响  

Effects of dexamethasone and vitamin B12 on expression of fibroblast growth factor 10 and fibroblast growth factor receptor 2b during early palatogenesis

作　　者：何苇[1,2] 卢胜军[1,3] 李承浩[1,3] 周京琳[1] 蒙田[1] 郑谦[1,3] 石冰[1,3] 

机构地区：[1]口腔疾病研究国家重点实验室四川大学,四川成都610041 [2]遵义医学院附属口腔医院口腔颌面外科,贵州遵义563000 [3]四川大学华西口腔医院唇腭裂外科,四川成都610041

出　　处：《华西口腔医学杂志》2010年第5期551-555,共5页West China Journal of Stomatology

基　　金：国家自然科学基金重点资助项目(30530730)

摘　　要：目的观察地塞米松和维生素B12作用后小鼠胚胎腭突成纤维生长因子10(Fgf10)及成纤维生长因子2b受体(Fgfr2b)信号的变化。方法将孕鼠分为致畸组、拮抗组和对照组,各组孕鼠分别注射地塞米松、地塞米松和维生素B12、生理盐水,胚胎12.5和13.5 d处死孕鼠并获取胚胎腭突,采用免疫印迹和BrdU染色的方法检测胚胎腭突Fgf10和Fgfr2b信号和间充质细胞增殖的变化。结果地塞米松作用后,小鼠胚胎腭突Fgf10和Fgfr2b表达显著下调,间充质细胞增殖抑制;维生素B12拮抗后,虽然Fgf10和Fgfr2b表达仍然下调,但间充质细胞增殖恢复。结论地塞米松和维生素B12影响小鼠胚胎腭突Fgf10和Fgfr2b表达和间充质细胞增殖,但二者的变化并不协调一致。Objective To observe the alteration of fibroblast growth factor 10（Fgf10） and fibroblast growth factor receptor 2（Fgfr2b） signal in mouse embryonic palate after dexamethasone and vitamin B12 exposure.Methods Dams were divided teratogenetic group,antagomistic group and control group and were respectively injected dexamethasone,dexamethasone and vitamin B12,and normal sodium.Dams were killed and fetus was collected at embryo 12.5 and 13.5 day.The expression of Fgf10 and Fgfr2b and mesenchymal cells proliferation of mouse embryonic by western blotting and BrdU assay were checked.Results Fgf10 and Fgfr2b expression was down-regulated and mesenchymal cells proliferation was inhibited significantly after dexamethasone exposure.After vitamin B12 treatment,Fgf10 and Fgfr2b expression did not restore,but cells proliferation was recovered.Conclusion Dexamethasone and vitamin B12 affected the expression of Fgf10 and Fgfr2b of mouse embryonic palate and mesenchyme cells proliferation,but the change was disaccord.

关 键 词：胚胎腭突 地塞米松 维生素B12 

分 类 号：R782.2[医药卫生—口腔医学]