翠菊‘花束绯红’叶片的植株再生体系建立  

Establishment of Plantlet Regeneration System from Leaves Explants for Callistephus chinensis‘Bouquet Scarlet'

作　　者：周索[1] 杜丽[1] 褚学英[1] 包满珠[2] 

机构地区：[1]南阳师范学院生命科学与技术学院,河南南阳473000 [2]华中农业大学园艺林学学院,教育部园艺植物生物学重点实验室,武汉430070

出　　处：《园艺学报》2010年第10期1667-1672,共6页Acta Horticulturae Sinica

基　　金：河南省自然科学基金项目(2009A210017);南阳师范学院STP项目(STP2009003)

摘　　要：以翠菊‘花束绯红’无菌苗为材料,研究了植物生长调节剂浓度及组合、光照条件、不同附加物对叶片及其愈伤组织再生植株的影响。结果表明:(1)在光照培养条件下,诱导翠菊叶片不定芽形成的最适培养基为MS+6-BA3.0mg·L-1+IBA1.0mg·L-1,诱导率为56.7%,平均不定芽数为3.3;(2)黑暗培养不利于叶片不定芽的分化,但促进叶片愈伤组织的形成;(3)培养基中分别添加脯氨酸、水解酪蛋白和NH4NO3等均能明显促进愈伤组织不定芽的诱导,最适培养基为MS+6-BA1.0mg·L-1+IBA0.1mg·L-1+脯氨酸300mg·L-1,诱导率为82.8%,平均不定芽数为4.5;(4)不定芽移到幼苗生根培养基(1/2MS+IBA0.1mg·L-1)上,生根率为89.1%,移栽后成活率达89.3%。Some factors including plant growth regulator concentrations and combinations,illumination conditions and different additives were studied on Callistephus chinensis‘Bouquet Scarlet＇ leaves for plantlet regeneration.The results showed that：（1）Under light conditions,the appropriate medium for adventitious bud induction from leaves of C.chinensis‘Bouquet Scarlet＇was MS ＋ 3.0 mg · L-1 6-BA ＋ 1.0 mg · L-1 IBA,the induction frequency was 56.7% and the adventitious bud number per explants was 3.3.（2）Although the adventitious buds from leaves didn＇t benefit differentiate in darkness conditions,it was accelerate the formation of the leaf callus.（3）Some substances in the medium with proline,CH and NH4NO3 could promote the adventitious bud induction from calli.The suitable medium was MS ＋ 1.0 mg · L-1 6-BA ＋ 0.1 mg · L-1 IBA ＋ 300 mg · L-1 proline,the induction frequency was 82.8% and the adventitious bud number per explants was 4.5.（4）On rooting induction medium（1/2MS ＋ 0.10 mg · L-1 IBA）,the rooting rates was 89.1% and the survial rates of regenerated plants was 89.3%.

关 键 词：翠菊 叶片 愈伤组织 植株再生 

分 类 号：S681[农业科学—观赏园艺]