小鼠大脑中动脉栓塞脑局部缺血模型及其评价方法  

Evaluation of Middle Cerebral Artery Occlusion Induced Focal Cerebral Ischemia Model in Mice

作　　者：于培兰[1] 隋雷鸣[1] 韩松[1] 赵焕英[1] 江君[2] 李俊发[2] 

机构地区：[1]首都医科大学医学实验与测试中心 [2]首都医科大学神经生物学系 北京神经科学研究所

出　　处：《首都医科大学学报》2010年第5期586-590,共5页Journal of Capital Medical University

基　　金：国家自然科学基金(30670782,30871219);国家重点基础研究发展计划(973项目)(2006CB5041);北京市自然科学基金A类项目(5072008);北京市教育委员会科技计划重点项目(KZ200810025012);北京市属高等学校人才强教计划项目(PHR200906116);北京市新世纪百千万人才工程培养经费(08-016)资助项目~~

摘　　要：目的通过比较客观和可靠的神经行为学评分,以及脑梗死体积和水肿率的计算,评价小鼠大脑中动脉阻塞(middlecerebral artery occlusion,MCAO)局部缺血模型的成功率和可靠性。方法线栓法致小鼠MCAO制备大脑局部缺血模型,应用2,3,5-氯化三苯基四氮唑(TTC)染色和神经行为学评分等方法,计算脑梗死体积、水肿率并观察行为学指标。结果TTC染色证实,MCAO可诱发明显的小鼠脑局灶性缺血,手术成功率约为90.8%,其中以皮质缺血为主,少数为单纯海马和丘脑缺血;脑局部缺血损伤可引起小鼠神经行为学的显著改变,即总体神经行为学评分增加;同时,脑梗死体积约占全脑体积的35%,分析结果准确和稳定。结论本研究成功地建立了MCAO致小鼠脑局部缺血的模型,且有较高的成功率(90.8%);同时所用评价神经功能损伤和脑梗死体积方法具有较高的可靠性。Objective To study the success rate and reliability of middle cerebral artery occlusion（MCAO） model by objective and reliable neurological score,infarct volume,and edema rate.Methods Cerebral ischemia model of MCAO was established in mouse using the suture method.Infarct volume,edema rate and behavioral performance was evaluated by 2,3,5-triphenyltetrazolium chloride（TTC） staining and neurological score.Results MCAO could induce obvious focal cerebral ischemia by TTC staining and the success rate of this model was about 90.8%.Besides,MCAO could induce three types of cerebral ischemia： cortical,hippocampal and thalamic ischemia.And the predominant type is cortical ischemia.MCAO-induced cerebral ischemia could significantly increase the neurological score compared with the sham group.The procedure for infarct volume evaluation of the MCAO model was accurate and stable;infarct volume was about 35% of entire brain volume.Conclusion Cerebral ischemia model of MCAO was established in mice successfully（90.8%）.The evaluation of neurological deficit and infarct volume was technically reliable.

关 键 词：大脑中动脉阻塞 神经功能评分 梗死体积 TTC染色 

分 类 号：R743.3[医药卫生—神经病学与精神病学]