急性早幼粒白血病细胞CD59表达缺失  

Acute Promyelocytic Leukemia with CD59 Deficiency

作　　者：魏辉 田征 王晓静 刘凯奇 张翠萍 王慧君 秘营昌 王建祥 

机构地区：[1]中国医学科学院协和医学院血液学研究所血液病医院,天津300020

出　　处：《中国实验血液学杂志》2010年第5期1105-1108,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目;编号30700419;天津市自然科学基金项目;编号08JCYBJC06400

摘　　要：CD59是糖基磷脂酰肌醇锚定蛋白,通过抑制膜攻击复合物保护细胞免受补体介导的溶细胞作用。本研究探讨急性早幼粒细胞白血病(APL)细胞CD59表达情况。应用流式细胞术检测19例急性早幼粒白血病细胞CD59的表达及NB4细胞经全反式维甲酸(ATRA)处理前后CD59的表达。用PCR方法扩增白血病患者和NB4细胞的pig-A基因编码序列并进行测序。结果表明,19例初治APL患者中12例CD59膜表达缺失,高于其他非APL细胞(40例non-APL中14例缺失,p=0.042)。对6例治疗前CD59表达缺失APL患者CR后再次检测CD59的表达,6例患者CD59的表达在CR后均恢复正常,这提示CD59的表达缺失只发生在早幼粒白血病细胞上。检测发现APL细胞系NB4的CD59表达也缺失,NB4细胞经ATRA处理后CD59的表达并没有随着细胞分化而出现变化。对NB4细胞和1例CD59表达缺失APL患者的pig-A基因编码序列测序显示,pig-A基因编码序列没有突变,因此APL的CD59表达缺失并不是由于pig-A基因的突变造成的。结论:APL细胞较多地有CD59表达缺失。CD59 is a glycosyl-phosphatidyl inositol-anchored protein with the capacity to block the formation of membrane-attack complex,and protect the cells from complement-mediated cytolysis.The study was aimed to investigate whether CD59 is deficient in acute promyelocytic leukemia（APL） blast cells.Expression of CD59 on APL blast cells was analysed by flow cytometry.Expression of CD59 on NB4 cells was determined by flow cytometry before and after treating with all trans retinoic acid（ATRA）.Pig-A gene coding region was sequenced.The results showed that the deficiency of CD59 expression in 12 out of 19 APL samples was found,its incidence was significantly higher than that in other acute myeloid leukemia（AML） samples（deficiency of CD59 expression in 14 of 40 non-APL AML samples,p=0.042）.The expression of CD59 became normal after the patients achieved complete remission（CR）,which indicated that the deficient of CD59 expression was only found in APL blast cells,but also found in APL cell line NB4 cells.The expression of CD59 was not changed after NB4 cells were induced to differentiate by ATRA.Sequencing pig-A gene coding region of NB4 cells and one APL patient with deficiency of CD59 displayed that the mutation of pig-A gene was not observed,therefore the deficiency of CD59 expression in APL cells did not result from mutation of pig-A gene.It is concluded that the deficiency of CD59 expression exists in APL blast cells more probably.

关 键 词：急性早幼粒细胞白血病 CD59 糖基磷脂酰肌醇锚定蛋白 pig-A基因 

分 类 号：R733.71[医药卫生—肿瘤]