针对c-myc基因的小干扰RNA对急性淋巴细胞白血病Jurkat细胞的c-myc、h-tert表达的影响  

Effects of Small Interference RNA against c-myc on Expression of c-myc and h-tert Genes in Acute Lymphoblastic Leukemia Cell Line Jurkat

作　　者：刘庭波[1,2] 骆晓峰[1] 陈志哲[1] 胡建达[1,2] 

机构地区：[1]福建医科大学协和医院血液科 [2]福建医科大学医学技术与工程学院,福建福州350001

出　　处：《中国实验血液学杂志》2010年第5期1151-1154,共4页Journal of Experimental Hematology

基　　金：福建省高校新世纪优秀人才计划;编号NCETFJ-0705

摘　　要：本研究探讨针对c-myc的小干扰RNA对急性淋巴细胞白血病Jurkat细胞c-myc,h-tert基因和蛋白表达的影响,为白血病的基因治疗提供新方法和靶点。针对c-myc mRNA的第1545-1565靶位点用化学合成法合成siRNA,经转染剂转染入Jurkat细胞。应用RT-PCR及Western blot分别检测c-myc siRNA作用前后c-myc、h-tert基因的mRNA及蛋白表达的变化。结果表明:c-myc siRNA能明显抑制Jurkat细胞的增殖,作用48小时的半数抑制浓度(IC50)约为75nmol/L。c-myc siRNA可引起Jurkat细胞的c-myc、h-tert mRNA和C-MYC、hTERT蛋白表达水平降低。结论:c-myc siRNA明显降低Jurkat细胞c-myc、h-tert基因的mRNA及蛋白表达水平。c-myc siRNA可能是通过抑制c-myc mRNA表达而降低胞内C-MYC蛋白水平。This study was purposed to investigate the effect of small interfering RNA against c-myc on c-myc,h-tert gene and protein expressions in acute lymphoblastic leukemia cell line（Jurkat cells）,so as to provide new methods and targets for gene therapy of leukemia.The siRNA against target sites 1545-1565 of c-myc mRNA was chemically synthesized and was transfected into Jurkat cells by transfectant.The c-myc,h-tert mRNA and protein expression levels before and after treatment with c-myc siRNA were detected by RT-PCR and Western blot,respectively.The results showed that c-myc siRNA obviously inhibited the proliferation of Jurkat cells,the half inhibitory concentration（IC50） for 48 hours was about 75 nmol/L.c-myc siRNA could decrease c-myc,h-tert mRNA and C-MYC,h-TERT protein expression levels of Jurkat cells.It is concluded that c-myc siRNA significantly reduce c-myc,h-tert mRNA and protein expression levels through inhibiting c-myc mRNA expression and decreasing intracellular level of C-MYC protein.

关 键 词：C-MYC基因 h-tert基因 急性淋巴细胞白血病 JURKAT细胞 小干扰RNA 

分 类 号：R733.7[医药卫生—肿瘤]