抗VEGF抗体联合重组人可溶性TRAIL诱导白血病细胞K562凋亡的研究  

Combined Effect of Anti-Vascular Endothelial Growth Factor Antibody and Recombinant Human TRAIL on Inducing Apoptosis of Leukemia K562 Cells

作　　者：王阿慧[1] 钟志敏[1] 周小棉[1] 

机构地区：[1]广州医学院附属市第一人民医院检验科,广东广州510180

出　　处：《中国实验血液学杂志》2010年第5期1163-1167,共5页Journal of Experimental Hematology

基　　金：广州市医药卫生科技项目(2008-YB-017;2008-YB-003;2009-zdi-10;2009-zdi-21);广东省自然基金项目(9151008901000190);国家自然基金项目(30870753)

摘　　要：本研究探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)和抗血管内皮生长因子(VEGF)抗体对人慢性髓系白血病细胞K562的生长抑制和诱导凋亡的协同作用。将TRAIL和抗VEGF抗体单独及联合作用于K562细胞,应用CCK-8法检测各组的细胞抑制率和用流式细胞仪检测各组的细胞凋亡率。结果表明:以TRAIL 75、100和150ng/ml的浓度作用于K562细胞48小时的凋亡率分别为(4.26±0.67)%、(8.91±0.55)%、(11.71±0.78)%;抗VEGF抗体2.5、5和7.5μg/ml的浓度作用于K562细胞48小时的凋亡率分别为(3.95±0.69)%、(7.98±0.74)%和(10.26±0.83)%。以抗VEGF抗体2.5μg/ml+TRAIL 75 ng/ml或抗VEGF抗体5μg/ml+TRAIL100 ng/ml或抗VEGF抗体7.5μg/ml+TRAIL150 ng/ml作用于K562细胞48小时的细胞凋亡率分别为(22.16±0.93)%、(36.32±1.31)%和(49.19±0.71)%。抗VEGF抗体与TRAIL联合作用于K562细胞后,细胞抑制率及凋亡率显著高于单独应用TRAIL组或抗VEGF抗体组(p<0.05)。结论:TRAIL和抗VEGF抗体对K562细胞在诱导凋亡过程中具有协同作用。The objective of this study was to investigate the synergistic effect of soluble human recombinant tumor necrosis factor related apoptosis inducing ligand（TRAIL） protein combined with anti-vascular endothelial growth factor（anti-VEGF） antibody on inducing apoptosis of leukemia K562 cells.The inhibitory rates and apoptotic rates of K562 cells treated with TRAIL and anti-VEGF antibody alone and their combination for 48 hours were examined by CCK-8 assay and flow cytometry respectively.The results indicated that the apoptotic rates of K562 cells induced with 75,100 and 150 ng/ml TRAIL after culture for 48 hours were（4.26±0.67）%,（8.91±0.55）% and（11.71±0.78）% respectively.The apoptotic rates of K562 cells induced with 2.5,5 and 7.5 μg/ml anti-VEGF antibody after culture for 48 hours were（3.95±0.69）%,（7.98±0.74）% and（10.26±0.83）% respectively.The apoptotic rates of K562 cells treated with combination use of 2.5 μg/ml anti-VEGF antibody and 75 ng/ml TRAIL,5 μg/ml anti-VEGF antibody and 100 ng/ml TRAIL,and 7.5 μg/ml anti-VEGF antibody and 150 ng/ml TRAIL for 48 hours were（22.16±0.93）%,（36.32±1.31）% and（49.19±0.71）% respectively.The combined use of above mentioned agents induced significantly higher apoptosis and cytotoxicity than that of TRAIL or anti-VEGF antibody alone（p0.05）.It is concluded that the combination use of TRAIL and anti-VEGF antibody can significantly increase the sensitivity of K562 cells to apoptosis.

关 键 词：TRAIL 抗VEGF抗体 白血病 细胞凋亡 

分 类 号：R733.7[医药卫生—肿瘤]