ZAP-70用于慢性淋巴细胞白血病标准化检测方法的探讨  

Evaluation of Standardized ZAP-70 Expression Detection in CLL——Review

作　　者：王银花[1] 徐卫[1] 李建勇[1] 

机构地区：[1]南京医科大学第一附属医院,江苏省人民医院血液科,江苏南京210029

出　　处：《中国实验血液学杂志》2010年第5期1354-1358,共5页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目(编号30871104,30971295,30971296);江苏省医学领军人才基金资助项目(编号LJ200623);江苏省医学重点人才基金资助项目(编号RC2007042);江苏省“青蓝工程”中青年学术带头人培养对象基金资助项目;江苏省“六大人才高峰”基金资助项目

摘　　要：慢性淋巴细胞白血病是一种临床预后差异极大的疾病。由于ZAP-70与免疫球蛋白重链可变区(IgVH)基因突变有明显相关性且检测方法相对简单,故ZAP-70成为IgVH基因突变的理想替代指标。近年来,不同的ZAP-70检测方法表现出的差异性限制了ZAP-70在临床上的应用,因此ZAP-70标准化检测方法的研究成为焦点。本文就ZAP-70的生物学特征、临床意义、检测方法进行综述。检测方法主要集中在分析方法和实验技术两方面;分析方法包括百分比法、荧光定量法、参比法,其中重要的一步是设定对照;实验技术包括标本的存储、检测时间、固定方法、抗体和荧光素的选择。虽然大量文献对检测方法的诸多变量研究进行了比较,但ZAP-70标准化检测方法在目前尚未有定论。Chronic lymphocytic leukemia（CLL） is a disease with variable clinical course.ZAP-70 expression shows a high concordance with IgVH gene mutational status and the method of detection for ZAP-70 expression is relatively simple,thus the ZAP-70 is used as a surrogate marker for IgVH gene mutational status.In recent years,ZAP-70 expression evaluation of different methods have shown difference that restrict the clinical application of ZAP-70.Therefore,the standardization of ZAP-70 expression detection has become a focus.The current review summarizes biocharacteristics,clinical application and detection method of ZAP-70.The detection methods of ZAP-70 are divided into analytical methods and experiment techniques.The analytical methods include percentage method,fluorescent quantitation and ratio method,in which the most important procedure is setting control.The experiment techniques include sample storage,time from blood draw to detection,fixation method,the antibody and the selection of fluorescence.Plenty of literatures have compared the variables,but the standardization of ZAP-70 expression detection method has not yet been decided.

关 键 词：慢性淋巴细胞白血病 ZAP-70 检测方法 

分 类 号：R733.72[医药卫生—肿瘤] Q503[医药卫生—临床医学]