宫颈癌组织中APC基因启动子区甲基化的初步研究  

Initial discussion of the value of APC gene promoter methylation in cervical cancer

作　　者：乔玉环[1] 赵晓丽[1] 刘红彦[1] 

机构地区：[1]郑州大学第一附属医院妇产科,郑州450052

出　　处：《现代妇产科进展》2010年第10期744-746,共3页Progress in Obstetrics and Gynecology

摘　　要：目的:探讨APC基因启动子区甲基化状态及其在宫颈癌发生发展中的意义。方法:采用甲基化特异性聚合酶链反应(methylation-specific PCR,MSP)方法分别检测15例正常宫颈上皮、60例宫颈鳞癌新鲜组织中APC基因启动子区的甲基化状态。结果:正常宫颈组织中未检测到APC基因甲基化片段,宫颈癌组织中APC基因甲基化阳性率65%,两者差异有统计学意义(P<0.05)。宫颈癌中不同临床分期甲基化阳性率差异有统计学意义(P<0.05),不同组织学分级、有无淋巴结转移组甲基化阳性率差异无统计学意义(P>0.05)。结论:APC基因启动子区异常甲基化在宫颈癌形成过程中是早期且频繁发生的事件,可能参与了宫颈癌的发生发展。Objective：To investigate methylation status of APC gene and its significance in the development of cervical cancer.Methods：The methylation status of APC gene were detected in 15 cases of normal cervical epithelium,60 cases of fresh cervical squamous cell carcinoma tissues by means of Methylation-specific polymerase chain reaction（methylation-specific PCR,MSP）.Results：The methylation rate of APC gene in cervical cancer was 65%,while no methylation of APC gene was found in normal cervical tissues.A comparison of the positive rate showed significant differences（P0.05）.The positive expression rate of MSP was significant difference in different clinical stages of cervical squamous cell carcinoma（P0.05）.The positive expression rate of lymph node metastasis and different histological grading were no significant difference（P0.05）.Conclusion：The promoter region of the APC gene methylation was an early and frequent event,and may be related with tumorigenesis of cervical cancer.

关 键 词：宫颈肿瘤 基因 APC 甲基化 

分 类 号：R737.33[医药卫生—肿瘤]