人肝癌细胞BEL-7402非常规照射模式生物效应探讨  

Preliminary Study of Radiobiological Effects on Human Hepatocarcinoma Cells BEL-7402 by Different Irradiation Model

作　　者：阮婷彦 楚建军[2] 金建荣[2] 周乐源[2] 李莉华[2] 张福正[2] 贺蓓娃[2] 赵涤非[2] 刘志辉[2] 丁阳[2] 

机构地区：[1]无锡人民医院肿瘤科,江苏无锡214000 [2]苏州大学附属无锡第四人民医院肿瘤科

出　　处：《肿瘤防治研究》2010年第10期1128-1131,1135,共5页Cancer Research on Prevention and Treatment

基　　金：江苏省科技厅社会发展指导性课题资助项目(BS2006504)

摘　　要：目的探讨不同照射模式对人肝癌细胞株BEL-7402生物效应变化影响及其与人肝细胞株QSG-7701放射敏感度差异。方法 6MVX线两种模式照射BEL-7402细胞(包括0、1、2、3、5、7、9、10Gy共8个吸收剂量点,剂量率3Gy/min):(1)急速照射组,照射完成时间0～4min;(2)模拟三维适形照射组:照射完成时间:12～15min。成克隆分析法计算存活分数,多靶单击数学模型拟合曲线,求出SF2、D0、Dq等参数值;6MVX线单次5Gy照射体外培养的BEL-7402、QSG-7701细胞,采用MTT比色法、流式细胞术测定细胞受照后不同时间存活率、凋亡率、细胞周期。结果 BEL-7402细胞模拟急速照射组和三维适形照射组D0、Dq、SF2值分别为1.78和1.69、1.45和1.54、0.625和0.654;QSG-7701细胞照后24h凋亡率最高(18%),而BEL-7402细胞照后12h凋亡率最高(32%),存活率最低(65%),两株细胞照后12h凋亡率和存活率差异最大(P<0.01)。结论三维适形照射模式下分次照射时间延长,生物效应下降;BEL-7402及QSG-7701两株细胞在照后存在放射敏感度差异。Objective To investigate the radiobiological effects of two irradiation protocols on human hepatocarcinoma cells BEL-7402 and hepatic cells QSG-7701.Methods BEL-7402 cells were irradiated by total doses of 0 Gy,1 Gy,2 Gy,3 Gy,5 Gy,7 Gy,9 Gy,10Gy at a dose rate of 3Gy/min.Two different delivery patterns were acute irradiation model with the fraction-delivery-time was 0～4 min;and 3-Dimensional conformal radiation therapy（3DCRT） model with the fraction-delivery-time was lasted 13～15 min.The surviving fractions were determined by classical colony forming assay.The radiobiological parameters were calculated,and cell survival curves were plotted according to ＂the single-hit multi-target model＂.The survivability,apoptosis and cell cycles of BEL-7402 and QSG-7701 cells irradiated by 6MV X-ray with a single dose of 5 Gy,were assessed by methyl thiazole tetrazolium（MTT） assay and flow cytometry（FCM）.Results The values of D0,Dq,SF2 of BEL-7402 cell line in acute irradiation pattern and 3DCRT irradiation pattern were 1.69 and 1.78,1.45 and 1.54,0.625 and 0.654 respectively.The number of apoptotic BEL-7402 cells reached a peak（32%） and showed a lowest survival（65%） at the 12h after irradiation,while irradiated apoptotic rate in QSG-7701 cells showed highest（18%） at the 24h.Apoptosis and survival between the two cell lines at 12h induced by radiation were significant different（P0.01）.Conclusion The radiobiological effectiveness were able to be influenced by prolonged fraction-delivery-time for 3DCRT.Radiosensitivity was markedly various from cancer and normal cellular line.

关 键 词：肝癌细胞 肝细胞 三维适形放射疗法 细胞存活曲线 放射敏感度 

分 类 号：R735.7[医药卫生—肿瘤]