黑灵芝多糖对体外培养的小鼠腹腔巨噬细胞功能的影响  

Effects of polysaccharide of Ganoderma atrum on the function of mouse peritoneal macrophages in vitro

作　　者：张莘莘[1] 李文娟[1] 聂少平[1] 谢明勇[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《中国药理学通报》2010年第9期1139-1142,共4页Chinese Pharmacological Bulletin

基　　金：国家高技术研究发展计划(863计划)资助项目(No2008AA10Z325);食品科学与技术国家重点实验室目标导向资助项目(NoSKLF-MB-200806)

摘　　要：目的研究黑灵芝多糖对小鼠腹腔巨噬细胞功能的影响。方法用不同浓度的黑灵芝多糖作用于正常的和LPS活化的腹腔巨噬细胞;测定巨噬细胞代谢MTT活力;Griess法测定NO的产生;ELISA法检测巨噬细胞培养上清中TNF-α、IL-1β的分泌水平。结果黑灵芝多糖对细胞代谢MTT活力有增强作用;在20～160mg·L-1范围内,多糖呈剂量依赖性地促进正常的巨噬细胞分泌NO、TNF-α、IL-1β,增强免疫;而巨噬细胞经LPS激活后,与LPS组比较,多糖不同程度地抑制NO、TNF-α、IL-1β的过量分泌。结论黑灵芝多糖能有效地增强小鼠腹腔巨噬细胞的免疫,可改善LPS对小鼠腹腔巨噬细胞的诱导作用。Aim To investigate the effects of polysaccharide of Ganoderma atrum（PSG-1） on the function of mouse peritoneal macrophages.Method Different concentrations of PSG-1 were added to the normal or LPS-activited peritoneal macrophages.Metabolic activity of peritoneal macrophages was measured by MTT assay.Nitric oxide was detected by Griess reagent.Concentrations of TNF-α and IL-1β in supernatants were determined by using quantitative sandwich enzymelinked immunosorbent assay（ELISA） kits.Results PSG-1 could enhance the metabolic activity of peritoneal macrophages.The production of NO,TNF-α and IL-1β was increased in a dose-depended manner when macrophages were exposed to polysaccharide of Ganoderma atrum ranging from 20 to 160 mg·L-1.After mouse peritoneal macrophages were induced by lipopolysaccharide,compared with the control group,polysaccharide of Ganoderma atrum could markedly inhibit the secretion of NO,TNF-α and IL-1β.Conclusion Polysaccharide of Ganoderma atrum can promote the immune function of mouse peritoneal macrophages and improve the adverse effect induced by lipopolysacchairde in mice.

关 键 词：黑灵芝多糖 巨噬细胞 免疫调节 抗炎 一氧化氮 肿瘤坏死因子-Α 白细胞介素-1Β 

分 类 号：R-332[医药卫生] R284.1