糖尿病大鼠肾脏水通道蛋白-2mRNA的表达及黄芪的调节作用  

The mRNA expression of renal aquaporin-2 in rats with diabetes mellitus and the therapeutical effect of astragalus membranaceus

作　　者：穆鑫[1] 袁霞[1] 康白[2] 何国荣[1] 杜冠华[1] 

机构地区：[1]中国医学科学院北京协和医学院药物研究所,北京100050 [2]潍坊医学院药理学教研室,山东潍坊261042

出　　处：《中国药理学通报》2010年第9期1176-1179,共4页Chinese Pharmacological Bulletin

基　　金：重大新药创制科技重大专项-综合性新药研究开发技术大平台(No2009YZH-LCH07);卫生部行业基金资助项目(No200802041)

摘　　要：目的观察黄芪注射液(astragalus membranaceus,AM)对链脲佐菌素(streptozotocin,STZ)诱导糖尿病(diabetes mellitus,DM)大鼠肾脏髓质水通道蛋白-2(aquaporin-2,AQP-2)基因表达的影响,以探讨黄芪治疗糖尿病的分子生物学机制。方法实验大鼠分为4组:正常组、糖尿病模型组、AM小剂量组和AM大剂量组,后两组应用AM5、10g·kg-1·d-1腹腔注射6周,测定各组大鼠血糖、血清胰岛素及C肽水平,用代谢笼法测定各组大鼠24h尿量,RT-PCR方法测定各组大鼠肾AQP-2 mRNA的表达。结果糖尿病大鼠血糖水平明显增高,血清胰岛素及C肽水平明显下降,单用黄芪注射液治疗对血糖、血清胰岛素和C肽水平无明显影响,但黄芪治疗可使糖尿病大鼠尿量明显增加。RT-PCR检测发现糖尿病大鼠肾髓质AQP-2 mRNA的表达明显上调,黄芪治疗可使其表达下降。结论黄芪治疗糖尿病利水消肿、改善水平衡代谢紊乱的作用可能与其降低肾髓质AQP-2 mRNA的表达有关。Aim To investigate the changes of renal medullary aquaporin-2（AQP-2） in rats with diabetes mellitus（DM） induced by streptozotocin（STZ） and the therapeutical effect of astragalus membranaceus（AM）.Methods Thirty diabetes mellitus rats induced by STZ were randomly divided into three groups：DM group,AM low dosage group（5 g·kg-1 ·d-1）,AM high dosage group（10 g · kg-1 · d-1）.Another 10 normal rats were taken as the control group.The drug was given by intraperitonial injection for 6 weeks.Blood glucose,serum insulin and C-Peptide were recorded.A 24 h urine collection through metabolic cage was made to measure urinary volume;the RT-PCR technique was used to determine the levels of aquaporin-2 mRNA expression in kidney medulla.Results After STZ treat-ment,diabetic rats showed higher glucose and lower insulin,C-Peptide levels as compared with control rats.AM treatment had no effect on blood glucose,serum insulin and C-Peptide,but it could significantly increase the urinary volume of diabetic rats.The expression of AQP-2 mRNA was up-regulated in renal medulla of diabetic rats,AM treatment could alleviate the exprssion of AQP-2 mRNA.Conclusion The therapeutical effect of AM on diabetes mellitus may be related to the significantly decreased expression of AQP-2 in the kidney medulla.

关 键 词：水通道蛋白-2 糖尿病 黄芪注射液 水代谢 尿量 RT-PCR 

分 类 号：R-332[医药卫生] R282.71