二烯丙基二硫下调cyclin D1和CDK4的表达诱导人鼻咽癌CNE2细胞G_1期阻滞  

Diallyl disulfide induced cell cycle arrest in G_1 phase by downregulation of cyclin D1 and CDK4 in human nasopharyngeal carcinoma CNE2 cells

作　　者：胡波[1,2] 邱青朝[2] 贺修培[3] 赵其辉[1] 贺修胜[2] 

机构地区：[1]湖南环境生物职业技术学院,湖南衡阳421005 [2]南华大学肿瘤研究所,湖南衡阳421001 [3]云南省第一人民医院耳鼻咽喉科,云南昆明650032

出　　处：《中国药理学通报》2010年第9期1203-1207,共5页Chinese Pharmacological Bulletin

基　　金：湖南省自然科学基金重点项目(No09JJ3071);湖南省教育厅重点项目资助课题(No08A060);湖南环境生物职业技术学院院长科研基金资助项目(NoZ08-02);云南省社会发展科技计划应用基础研究资助项目(No2009ZC168m)

摘　　要：目的研究二烯丙基二硫(DADS)对人鼻咽癌CNE2细胞的影响及其分子机制。方法采用MTT法检测DADS对CNE2细胞增殖抑制作用;流式细胞术分析DADS对CNE2细胞周期分布的影响;运用RT-PCR和Western blot方法分析DADS作用CNE2细胞后,cyclin D1和CDK4的表达变化。结果 MTT结果显示,不同浓度DADS(90、140、240、400μmol·L-1)处理CNE2细胞48h后,生长抑制率分别为4.0%、13.8%、25.8%、51.2%;流式细胞术分析显示,DADS阻滞CNE2细胞于G1期,并呈浓度依赖性;RT-PCR和Western blot结果表明,细胞周期调控基因cyclinD1、CDK4表达下调。结论 DADS对CNE2细胞的增殖抑制作用与其阻滞细胞G1期有关,并且可能是通过抑制cyclin D1、CDK4的表达使CNE2细胞阻滞于G1期。Aim To investigate the effect of diallyl disulfide（DADS） on human nasopharyngeal carcinoma CNE2 cell line and its underlying molecular mechanism.Motheds The antiproliferative effect of DADS in different concentrations on CNE2 cell line was measured by MTT assay.Cell cycle analysis was analyzed by flow cytometry.The expression levels of cyclin D1and CDK4 were detected by RT-PCR and Western blot.Results The MTT assay showed that DADS had potent antiproliferative effect on CNE2 in a dose-dependent manner.After 90,140,240 and 400 μmol·L-1 DADS treatment for 48 hours,the viabilities of CNE2 cells were inhibited by 4.0%,13.8%,25.8% and 51.2%,respectively（P 0.05）.Flow cytometry analysis revealed that CNE2 cells of G1 phase were arrested after exprosed to different concentrations of DADS.The expression levels of cyclin D1,CDK4 were decreased significantly after treated with DADS in a dose-dependent manner using RT-PCR and Western blot.Conclusion The antiproliferative effect of DADS in CNE2 cell line is related to G1 phrase arrest through decreasing expression levels of cyclin D1 and CDK4.

关 键 词：二烯丙基二硫 鼻咽癌 CNE2细胞 细胞周期 CYCLIN D1 CDK4 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R329.28[医药卫生—基础医学]