一株有抑菌活性海洋放线菌的筛选及鉴定  

Screening and Identification of A Marine Actinomycete with Antibacterial Activity

作　　者：李鹏[1] 高鹏[1] 王健鑫[1] 

机构地区：[1]浙江海洋学院海洋科学学院,浙江舟山3160001

出　　处：《生物技术》2010年第5期50-53,共4页Biotechnology

基　　金：浙江省教育厅计划项目("舟山沿海潮间带微生物区系调查与应用的初步研究";20061133);舟山市科技计划项目("海水养殖环境微生物态制剂的研制和开发";06226);校级项目("动物肠道菌群降解几种类黄酮物质的研究";X05LQ02)资助

摘　　要：目的:筛选具有抑菌活性的海洋放线菌。方法:以金黄色葡萄球菌、大肠杆菌、白假丝酵母、黑曲霉、苏云金芽孢杆菌、副溶血弧菌、胶质芽孢杆菌作为指示菌,采用滤纸片法进行筛选,将筛选到的菌株进行培养特征研究、生理生化试验及16S rDNA序列分析,并将测序结果提交至NCBI进行相似性检索,进而绘制出系统进化树,得到鉴定结果。结果:从舟山海域分离到1株具有较好抑菌活性的放线菌菌株a10,鉴定结果显示a10是一株浅玫瑰色链霉菌突变株。Objective：To screen marine actinomycetes with antibacterial activity.Method：To Staphylococcus aureus,Escherichia coli,Canidia albicans,Aspergillus niger,Bacillus thuringiensis.V.parahaemolyticus and B.mucilaginosus as the indicator bacteria,strains were screened by filter paper method.Observing the cultured characteristics,physiological and biochemical results of the screened strain a10.The results of 16S rDNA sequence analysis were submitted to NCBI,then drawing phylogenetic trees.Identification results were obtained.Result：Isolating from the Zhoushan sea area with a good antibacterial activity of actinomycetes strain a10.The strain a10 was one kind variants strain of Streptomyces roseus.

关 键 词：滤纸片法 活性物质 16SrDNA 系统进化树 

分 类 号：Q939.132[生物学—微生物学]