染料木素促成骨样细胞增殖作用及其机制研究  

Effect and mechanism of genistein on the proliferation of MC3T3-E1 cells

作　　者：牛银波[1] 李宇华[2] 黄海涛[2] 孔祥鹤[1] 王婷梅[1] 梅其炳[1,2] 

机构地区：[1]西北工业大学生命科学院空间生物实验模拟技术国防重点学科实验室,陕西西安710072 [2]第四军医大学药学系药理学教研室,陕西西安710032

出　　处：《中国药理学通报》2010年第10期1309-1312,共4页Chinese Pharmacological Bulletin

基　　金：国家高技术研究发展计划(863计划)资助项目(No2008AA12A220)

摘　　要：目的观察染料木素(genistein)对成骨样细胞MC3T3-E1细胞增殖及骨形成蛋白-2(BMP-2)、β-连环素(β-catenin)表达的影响。方法培养成骨样细胞MC3T3-E1,加入不同浓度的染料木素(1×10-10、1×10-9、1×10-8、1×10-7、1×10-6和1×10-5mol·L-1),以1×10-9mol·L-1雌激素(β-estradiol,E2)作为阳性对照组,采用MTT比色试验法和碱性磷酸酶(ALP)活性检测法,观察染料木素作用后MC3T3-E1的增殖及分化情况,以Westernblot方法检测成骨样细胞中BMP-2和β-catenin蛋白的表达。结果染料木素可促进MC3T3-E1细胞增殖,染料木素作用后细胞的ALP值也明显升高,呈浓度依赖性;染料木素可增加BMP-2和β-catenin表达,且呈浓度依赖性。结论染料木素促进MC3T3-E1细胞增殖与分化,可通过调节BMP-2和Wnt/β-catenin信号通路而影响成骨样细胞的活性。Aim To investigate the effect of genstein on the proliferation and differentiation of MC3T3-E1 osteoblast cells as well as the expression of BMP-2 and β-catenin on protein levels.Methods Genistein was administrated to MC3T3-E1 cells at the concentrations of 1×10 -10,1×10-9,1×10-8,1×10-7,1×10-6 and 1×10 -5 mol·L -1 in DMEM.β-estradiol at the concentration of 1×10 -9 mol·L -1 was used as a positive control.And the effect on osteoblast proliferation and differentiation were assessed by MTT and ALP assays;the protein levels of BMP-2 andβ-catenin in MC3T3-E1 were evaluated by Western blot.Results Proliferation of MC3T3-E1 was promoted by different concentrations of genistein;Results of Western blot examination revealed that genistein increased BMP-2 and β-catenin expression in a dose-dependent manner. Conclusions The results indicate that genistein can promote proliferation and differentiation in MC3T3-E1 by increasing BMP-2 expression and enhancing Wnt/βcatenin signal pathway.

关 键 词：染料木素 成骨样细胞MC3T3-E1 增殖 分化 骨形态发生蛋白-2 Β-连环素 

分 类 号：R-332[医药卫生] R284.1