通心络对兔缺氧耐受性的影响  

Effects of Tongxinluo on hypoxia tolerance of rabbit

作　　者：吴相春 来静[3] 吴相锋 贾振华 王洪涛 唐思文 

机构地区：[1]河北以岭医药研究院 [2]国家中医药管理局重点研究室(心脑血管络病),河北石家庄050035 [3]石家庄市第三医院,河北石家庄050017

出　　处：《中国药理学通报》2010年第10期1383-1386,共4页Chinese Pharmacological Bulletin

基　　金：国家重点基础研究发展计划(973计划)资助项目(No2005CB523301)

摘　　要：目的观察通心络对兔急性缺氧耐受性的作用。方法 24只新西兰大耳白家兔随机分为对照组、缺氧组和通心络组,先在11.4%氧环境下低氧实验,60min后进行密闭缺氧,观察兔密闭缺氧耐受时间,ELISA法检测血清缺氧诱导因子1-α(HIF-1α)的含量、Western blot法检测主动脉组织HIF-1α和血管内皮生长因子(VEGF)的蛋白表达。结果与缺氧前比较,缺氧组与通心络组血清HIF-1α含量明显增加(P<0.01或P<0.05)。与对照组比较,缺氧组和通心络组主动脉组织HIF-1α和VEGF表达明显增强(P<0.01或P<0.05)。与缺氧组比较,通心络组家兔密闭缺氧耐受时间明显增加(P<0.05),血清HIF-1α含量明显增加(P<0.01),主动脉组织HIF-1α表达增强,VEGF表达明显增强(P<0.05)。结论 HIF-1α和VEGF的表达增高可能是缺氧适应的一种机制,通心络能提高兔的缺氧耐受性,其机制可能与上调血清及组织的HIF-1α和VEGF有关。Aim To investigate the effects of Tongxinluo（TXL,a Chinese medicine） on the hypoxia tolerance of rabbits with acute hypoxia and to explore the mechanism.Methods Twenty-four healthy flap-eared white rabbits were randomly divided into 3 groups：control group,hypoxia group and TXL group.The rabbits were in low oxygen condition of 11.4%O2 at first.After 60 minutes,air supply was closed.The hypoxia tolerance time of the rabbits was measured.The content of serum hypoxia inducible factor 1alpha（HIF-1α） was detected with ELISA.The expression of HIF-1αand vascular endothelial growth factor（VEGF） in the aorta of rabbit were detected by Western blot.Results Compared with hypoxia before,the serum HIF-1αof hypoxia and TXL groups increased significantly（P0.05 or P 0.01） .Compared with the control group,the HIF-1α and VEGF in the aorta of hypoxia and TXL groups expressed obviously（P0.05 or P0.01） .Compared with hypoxia group,the hypoxia tolerance time of TXL group increased significantly（P0.01） ,the serum HIF-1αcontent increased significantly at 60 minutes（P 0.01） ,and the expression of HIF-1αand VEGF in aorta tissue was enhanced obviously（P0.05） .Conclusions These results indicate that hypoxia can induce HIF-1αand VEGF by itself to adaptation.TXL can improve the hypoxia tolerance of rabbits.The impossible mechanism may be related to the up-regulation of HIF-1αand VEGF in serum and aorta tissue.

关 键 词：通心络 兔 低氧 缺氧耐受 低氧诱导因子1Α 血管内皮生长因子 

分 类 号：R-332[医药卫生] R287