Identification of zRAP55,a gene preponderantly expressed in StagesⅠandⅡ oocytes of zebrafish  

在斑马鱼Ⅰ和Ⅱ期卵母细胞中优势表达基因zRAP55的鉴定(英文)

作　　者：赵彩莲[1,2] 杨启文[2] 胡珈瑞[3] 叶鼎[2] 龚午鸣[2] 吕海英[1] 徐宗芸[2] 张训蒲[1] 宋平[2] 

机构地区：[1]华中农业大学水产学院,武汉430070 [2]武汉大学生命科学学院分子遗传与发育实验室,武汉430072 [3]武汉大学中南医院妇产科,武汉430071

出　　处：《Zoological Research》2010年第5期469-475,共7页动物学研究（英文）

基　　金：supported by grants from the National Basic Research Program of China(2004CB117400);the National Natural Science Foundation of China(30150005;30270675)~~

摘　　要：In an in silico search for gonand specific expressed genes, we have identified zRAP55 which is enriched in the ovary of zebrafish . zRAP55 encodes a protein of 382 amino acids with a highly conserved Lsm domain. zRAP55 protein shares more than 56% identities with that of other vertebrate species. RT-PCR results show that it is predominantly expressed in the ovary. In situ hybridization and immunohistochemistry studies reveal that zRAP55 is ubiquitously dispersed throughout the cytoplasm of stages Ⅰ and Ⅱ oocytes, whereas no expression is observed in stages Ⅲ and Ⅳ oocytes. As an RNA associated protein, zRAP55 might function in the control of protein translation at the early stages of oogenesis in zebrafish.通过性腺特异性表达基因的筛选,作者发现了一个在斑马鱼卵巢中富集的基因zRAP55。zRAP55蛋白由382个氨基酸组成并含有一个高度保守的Lsm区。zRAP55蛋白与其他脊椎动物一致性在56%以上。RT-PCR结果表明,zRAP55优势表达于卵巢中。原位杂交和免疫组织化学结果表明:在Ⅰ期和Ⅱ期卵母细胞中,zRAP55的阳性信号强烈,均匀地分布于整个细胞质中,但是在Ⅲ期和Ⅳ期卵母细胞中均检测不到信号。作为一个RNA相关蛋白,zRAP55可能在早期卵母细胞中具有调节蛋白质翻译的重要作用。

关 键 词：Lsm domain zRAP55 OOGENESIS IMMUNOHISTOCHEMISTRY ZEBRAFISH 

分 类 号：Q343[生物学—遗传学]