三氯乙烯对豚鼠脾和外周血淋巴细胞凋亡基因表达水平的影响  

mRNA expression of apoptosis genes in spleen and peripheral blood of guinea pigs treated with trichloroethylene

作　　者：刘月峰[1] 徐新云[1] 李学余[1] 周丽[1] 袁建辉[1] 杨细飞[1] 杨淋清[1] 

机构地区：[1]深圳市疾病预防控制中心,深圳518020

出　　处：《癌变．畸变．突变》2010年第5期379-382,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：深圳市科技计划项目(200802073)

摘　　要：目的:探讨三氯乙烯(trichloroethylene,TCE)对豚鼠脾和外周血淋巴细胞凋亡基因Bcl-2、BAX、BAD mRNA表达的影响。方法:采用豚鼠最大值法(guinea pig maximizationtest,GPMT)将18只豚鼠随机分为TCE实验组、阴性对照组、阳性对照组,共3组,每组6只。实验采用皮内注射的方式致敏,TCE实验组注射5%TCE(TCE∶橄榄油=5∶95),阳性对照组注射0.125%2,4-二硝基氯苯(2,4-dinitrochlorobenzene,DNCB)和福氏完全佐剂(Freund's adjuvant complete,FCA)等量混合物,阴性对照组注射橄榄油。各组于第1天皮内注射受试物致敏,第8天用受试物涂皮诱导,第15天涂皮激发。于第15天激发后24h,抽取豚鼠外周血,并处死豚鼠摘取脾脏。用荧光定量PCR检测脾和外周血淋巴细胞凋亡基因Bcl-2、BAX和BAD的mRNA表达水平。结果:阳性对照组和TCE实验组动物均出现明显皮肤损害,TCE致豚鼠皮肤变态反应动物模型建立成功。TCE实验组和阳性对照组豚鼠脾淋巴细胞Bcl-2、BAX和BAD的mRNA表达水平均较阴性对照组显著升高(P<0.01);但TCE实验组和阳性对照组豚鼠外周血淋巴细胞Bcl-2、BAX和BAD的mRNA表达水平与阴性对照组比较差异无统计学意义(P>0.05)。结论:三氯乙烯可诱导豚鼠产生明显的皮肤变态反应,对豚鼠脾淋巴细胞凋亡基因表达水平有一定影响。OBJECTIVE：To study mRNA expression of apoptosis genes Bcl-2,BAX,BAD in spleen and peripheral blood of guinea pigs after treatment with trichloroethylene （TCE）. METHODS： Guinea pig maximization test （GPMT） was applied in this study,18 guinea pigs were randomly divided into 3 groups,namely negative control,positive control and TCE treatment. Animals of the 3 groups were treated with olive oil,2,4-dinitrochlorobenzene （DNCB）,and TCE,respectively,by intradermal injection. Spleens and peripheral blood were collected from the animals for mRNA expression assay,Bcl-2,BAX,BAD in lymphocytes of spleen and peripheral blood were measured by real-time fluorescent PCR assay. RESULTS： Obvious skin damage was observed in the groups of positive control and TCE treatment,the skin damage included erythema and edema. The levels of Bcl-2,BAX and BAD mRNA expression increased significantly in spleen lymphocytes in positive control and TCE treatment groups. However,the levels of Bcl-2,BAX and BAD mRNA did not elevate significantly in peripheral blood lymphocytes in positive control and TCE treatment groups,when compared with negative control. CONCLUSION： Trichloroethylene could induce skin allergic reaction in guinea pigs,it also had effects on mRNA expressions of Bcl-2,BAX,BAD in spleen lymphocytes of guinea pigs.

关 键 词：三氯乙烯 豚鼠 脾 外周血 淋巴细胞 凋亡基因 

分 类 号：R730.45[医药卫生—肿瘤]