EB病毒胸腺嘧啶激酶抗体在鼻咽癌血清学诊断中的作用  

Development of new methods for the diagnosis of nasopharyngeal carcinoma

作　　者：皮国华 程道胜[3] 周逸 张文龙 

机构地区：[1]北京麦克莱金生物技术有限公司,100176 [2]北京新兴四寰生物技术有限公司 [3]广东省江门市中心医院检验科

出　　处：《中华实验和临床病毒学杂志》2010年第1期65-67,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的 建立EB病毒感染和鼻咽癌血清学检测的敏感,特异和有效的方法.方法 以EB病毒早期蛋白胸腺嘧啶激酶为检测抗原,建立ELISA和免疫纤维膜条方法,检测血清中的特异性的IgG抗体.结果 用ELISA和诊断条同时检测了401份血清,其中鼻咽癌患者血清127份.两种方法检测鼻咽癌患者血清抗体均阳性,274份门诊患者血清中,55份ELISA和诊断条检测抗体均阳性,3份诊断条方法检测阳性的血清,ELISA检测A值接近临界值.对胸腺嘧啶激酶的抗体阳性率明显地高于早期蛋白P54.结论 以胸腺嘧啶激酶为检测抗原,为鼻咽癌血清学诊断和高危人群的筛查提供更有效的手段.Objective Development of new methods, ELISA and immunostrip test, for the diagnosis of nasopharyngeal carcinoma. Methods The engineering purified antigens coat plate or absorb on nitrocellulose filter. The plate and diagnostic strips carrying antigens were used for detection of IgG antibody in the sera from nasopharyngeal carcinoma patients and outpatients patients. Results 127 cases sera from nasopharyngeal carcinoma patients were parallel detected TK/ IgG antibody by ELISA and immunostrips. The TK/IgG antibody are all positive in the 127 cases of nasopharyngeal carcinoma patients. 55 cases show positive by ELISA, 58 cases positive by immunostrips in 247 cases sera from outpatient. The antibody positive rate to early antigen p54 lower then to TK. Conclusion ELISA and imuunostrips are sensitive and specific means for detection of the IgG antibody to TK of EBV and the diagnosis of nasopharyngeal carcinoma.

关 键 词：疱疹病毒4型 人 胸腺嘧啶 血清学试验 鼻咽肿瘤 

分 类 号：R73[医药卫生—肿瘤]