Wnt5a、MMP2和MMP14在胃癌中的表达及其对临床病理特征的影响  

Expressions of Wnt5a,MMP2,and MMP14 in Gastric Cancer and Their Influences on Clinicopathologic Features

作　　者：江州华[1] 吴生华[1] 李小强[2] 俞继卫[1] 姜波健[1] 

机构地区：[1]上海交通大学医学院附属第三人民医院普外科,上海201900 [2]上海交通大学医学院附属第三人民医院病理科,上海201900

出　　处：《中国普外基础与临床杂志》2010年第10期1077-1082,共6页Chinese Journal of Bases and Clinics In General Surgery

基　　金：上海交通大学医学院科技基金项目(项目编号:2007XJ032项目编号:2009XJ21037)~~

摘　　要：目的研究Wnt5a、MMP2及MMP14在胃癌原发灶中的表达,探讨三者之间的相关性及其与临床病理特征的关系。方法免疫组化染色法检测106例胃癌手术标本及其中的39例正常胃组织中Wnt5a、MMP2和MMP14的表达,2χ检验和非参数检验分析三者间的关系及其与临床病理参数的关系。结果 Wnt5a、MMP2及MMP14在胃癌原发灶中广泛表达,均明显高于正常胃组织中的表达(P<0.05)。Wnt5a阳性表达者的肿瘤直径更大、浸润深度更深、区域淋巴结转移程度更高、TNM分期更晚、淋巴结转移率更高、存在淋巴管和血管浸润(P<0.05)。MMP2和MMP14阳性表达者的浸润深度更深、区域淋巴结转移程度更高、TNM分期更晚及淋巴结转移率更高(P<0.05),且均存在淋巴管浸润(P<0.05),但均与血管浸润与否无关。此外,MMP2阳性表达者的肿瘤直径更大(P<0.05)。Spearman秩相关分析显示,Wnt5a和MMP2(rs=0.240,P=0.014)、Wnt5a和MMP14(rs=0.251,P=0.010)、MMP2和MMP14(rs=0.444,P=0.000)两两之间表达均存在正相关性。结论 Wnt5a、MMP2及MMP14的表达或能促进胃癌侵袭和转移,且三者之间的表达呈正相关。Objective To study the expressions of Wnt5a,MMP2,and MMP14 in the primary lesions of gastric cancer and the influences on clinicopathologic features.Methods The expressions of Wnt5a,MMP2,and MMP14 in the specimens of 106 patients with gastric cancer and 39 patients from the adjacent normal gastric tissues were detected by immunohistochemical staining,χ2 test and non-parametric test were used to analyze the relationships among them and between them and their influences on the clinicopathologic features.Results Extensive expressions of Wnt5a,MMP2,and MMP14 were demonstrated in the gastric cancer,which were significantly higher than those in the normal gastric tissues respectively（P0.05）.Positive expression of Wnt5a was associated with larger tumor diameter,deeper depth of invasion,higher degree of regional lymph node metastasis,later TNM stage,and higher rate of lymph node metastasis（P0.05）.In addition,Wnt5a expression was also associated with lymphatic infiltration and vascular infiltration（P0.05）.The expressions of MMP2 and MMP14 were associated with lymphatic infiltration,but not with vascular infiltration.Higher expressions of MMP2 and MMP14 were correlated with deeper tumor invasion,higher degree of regional lymph node metastasis,later TNM stage,and higher rate of lymph node metastasis（P0.05）.In addition,higher expression of MMP2 possesed greater tumor diameter（P0.05）.Spearman rank correlation analysis revealed the positive relation between Wnt5a and MMP2（rs=0.240,P=0.014）,Wnt5a and MMP14（rs=0.251,P=0.010）,as well as MMP2 and MMP14（rs=0.444,P=0.000）. Conclusion Higher expressions of Wnt5a,MMP2,and MMP14 seem to promote invasion and metastasis of gastric cancer,and there are positive relations among their expressions.

关 键 词：胃癌 侵袭 转移 上皮间质转型 WNT5A 基质金属蛋白酶2 基质金属蛋白酶14/MT1-MMP 

分 类 号：R735.2[医药卫生—肿瘤]