高效液相色谱法测定金铃子滴丸中延胡索乙素的含量  

Determination of the Content of Tetrahydropulmatine in Fructus Toosendan Drop Pill with HPLC

作　　者：张妍[1] 王颖[1] 梅耀军[1] 郑平[1] 王文忠[1] 

机构地区：[1]天津商业大学生物技术与食品科学学院,天津300134

出　　处：《世界中西医结合杂志》2010年第10期861-862,共2页World Journal of Integrated Traditional and Western Medicine

摘　　要：目的建立测定金铃子滴丸中延胡索乙素含量的高效液相色谱法。方法采用HPLC法测定延胡索乙素含量,色谱柱为依利特C18(4.6mm×200mm,5μm);流动相为甲醇∶0.1%磷酸溶液(三乙胺调pH值至6.0)(55∶45);检测波长为280nm,流速为1.0mL·min-1,柱温为30℃,进样量10μL。结果延胡索乙素在0.01～0.20mg.mL-1范围内质量浓度与峰面积呈良好的线性关系,相关系数r=0.9999(n=7);平均回收率为100.25%,RSD为1.71%(n=6);重复性实验的相对标准偏差RSD为1.87%(n=6)。结论该测定方法重复性好、灵敏度高并且简便、准确,可作为金铃子滴丸中延胡索乙素含量的测定方法。Objective：To establish a method of HPLC for determining the content of tetrahydropulmatine in fructus toosendan drop pill.Methods：HPLC was adopted to determine the content of tetrahydropulmatine,with Elite C18 column（4.6 mm×200 mm,5 μm）and mobile phase,methanol：0.1% phosphoric acid（pH value of triethylamine：6.0）（55∶45）,at 280 nm;flow rate,1.0 mL·min-1,at 30 ℃ of column temperature and 19 μL of sample input.Results：The good linear relation between quality concentration and peak area for tetrahydropulmatine presented in the range from 0.01 to 0.20 mg·mL-1,and the relation factor（r）= 0.999 9（n=7）.The average recovery was 100.25% and RSD was 1.71%（n=6）.RSD of repeatable experiment was 1.87%（n=6）.Conclusion：The method is good in repeatability,high in sensitivity,sample and accurate in operation,which is suitable for the determination of the content of tetrahydropulmatine in fructus toosendan drop pill.

关 键 词：金铃子滴丸 延胡索乙素 高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学]