高脂日粮对孕鼠胎盘养分转运及胎鼠宫内生长的影响  

EFFECTS OF MATERNAL HIGH-FAT DIET ON PLACENTAL NUTRIENT TRANSFER AND INTRAUTERINE FETAL GROWTH IN RATS

作　　者：卓勇[1] 吴德[1] 林燕[1] 方正锋[1] 车炼强[1] 

机构地区：[1]四川农业大学动物营养研究所,雅安625014

出　　处：《营养学报》2010年第5期451-455,共5页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30471257);教育部创新团队计划资助项目(No.IRT0555)

摘　　要：目的研究孕鼠妊娠期采食高脂日粮对胎盘养分转运、胎鼠宫内生长、胎鼠肝脏能量和甲基化代谢相关基因表达量的影响。方法母鼠妊娠后分别采食高脂和低脂日粮,妊娠20d收集母体血清、胎盘、胎鼠肝脏。检测母体血清代谢底物和瘦素浓度,通过RT-PCR方法检测胎盘葡萄糖和氨基酸转运载体的表达量,胎盘信号传导与转录活化因子-3(STAT3),以及胎鼠肝脏甲基转移酶和能量代谢相关基因的mRNA表达量。结果孕鼠采食高脂肪日粮显著提高胎鼠宫内生长速度,血清甘油三酯和瘦素浓度,且显著上调胎盘STAT3、葡萄糖转运载体Slc38a2的mRNA表达量。高脂日粮使胎鼠肝脏甲基转移酶DNMT1基因表达量上调,而使DNMT3a的基因表达量下调,并使胎鼠肝脏过氧化物酶增殖子激活受体γ(PPARγ)、糖皮质激素受体(GR)、Na-K-ATP酶(Na-K-ATP)mRNA表达量有提高趋势。结论孕鼠采食高脂日粮可能通过瘦素-STAT3途径上调养分转运载体的表达量促进胎鼠的宫内生长,胎鼠宫内生长加快会导致肝脏能量代谢和DNA甲基化相关基因表达量发生改变。Objective To investigate the effects of maternal high-fat diet on placental nutrients transfer,fetal growth in utero and gene expressions in fetal liver.Method Pregnant rats were fed high-and low-fat diets until serum,placenta and fetal liver were collected on D 20 of pregnancy.The serum concentrations of metabolites and leptin were determined,and mRNA expression of placental nutrient transporter of glucose and amino acids,signal transducer and activator of transcription 3（STAT3）,fetal liver DNA methyltransferases-1 and 3a（DNMT1 and DNMT3a）,peroxisome proliferator-activated receptor-γ（PPAR-γ）,glucocorticoid receptor（GR） and Na-K-ATPase（Na-K-ATP）were detected by RT-PCR.Results High-fat diet promoted fetal growth,elevated serum concentrations of triglyceride and leptin.RT-PCR quanti-fication revealed high fat diet up-regulated placental STAT3 and Slc38a2 expressions.High-fat diet up-regulated DNMT 1,but down-regulated DNMT3a expression in fetal liver,and have the trend to increase PPAR-γ,GR and Na-K-ATP expressions.Conclusion High-fat diet up-regulated placental nutrient transfer to promote intrauterine fetal growth possibly through leptin-STAT3 pathway,and enhanced intrauterine fetal growth was associated with changed gene expressions related to energy metabolism and DNA methylation.

关 键 词：高脂日粮 胎盘养分转运 胎儿宫内生长 DNA甲基化 孕鼠 

分 类 号：R151.1[医药卫生—营养与食品卫生学]