17β-雌二醇对体外培养牛输卵上皮细胞中环氧合酶-2的表达影响  

EFFECTS OF 17β-ESTRADIOL ON THE EXPRESSION OF CYCLOOXYGENASE-2 IN BOVINE OVIDUCT EPITHELIAL CELLS

作　　者：武岳[1] 苏日娜[1] 付改玲[1] 曹金山[1] 曹贵方[1] 

机构地区：[1]内蒙古农业大学兽医学院,呼和浩特010020

出　　处：《内蒙古农业大学学报（自然科学版）》2010年第3期20-23,共4页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：内蒙古农业大学博士启动基金(201-206048)

摘　　要：目的:阐明17β--雌二醇(17β—estradiol,E2)对体外培养的奶牛输卵管上皮细胞(bovine oviduct epithelialcells,BOECs)中环氧合酶-2(cyclooxygenase-2,COX-2)基因表达的影响。方法:分离培养BOECs,建立BOECs培养体系作为体外试验模型,分别在培养液中添加10 pg/mL的E2分别培养2h、4h、6h,提取细胞总RNA及细胞总蛋白,利用实时荧光定量PCR,Western blot等实验技术检测COX-2 mRNA和蛋白的表达量。结果:输卵管上皮细胞的COX-2基因表达随添加E2培养液增加2h、4h明显高于对照组。结论:试验数据显示在体外培养的BOECs中E2影响牛输卵上皮细胞中COX-2的表达。For clarify regulatory effects of 17β——estradiol（E2） in cyclooxygenase-2（COX-2） of the bovine oviduct epithelial cells（BOECs）,the cultured BOECs were treated with E2（10 pg/ml） for 2,4 and 6 h respectively,and the expression of COX-2 mRNA and protein were determined by real time PCR and Western blot methods.The results showed that E2 significantly increased expression of COX-2 mRNA and protein in after 2h and 4h treated that compared with control.These results suggest the E2 regulation of expression of COX-2 mRNA and protein in BOECs.

关 键 词：牛输卵上皮细胞 17Β-雌二醇 环氧合酶合-2 实时荧光定量PCR WESTERN BLOT 

分 类 号：S823[农业科学—畜牧学] S852.16[农业科学—畜牧兽医]