骨肉瘤细胞核因子кB免疫组化研究  

Immunohistochemical Study N of nuclear Factor кB(NF-кB)in Osteosarcoma

作　　者：王东[1] 李颖嘉[1] 牟江洪[1] 陈俐 

机构地区：[1]第三军医大学野战外科研究所病理科,重庆400042

出　　处：《重庆医学》1999年第3期165-167,共3页Chongqing medicine

摘　　要：细胞核因子ＮＦ－кＢ在肿瘤血管生成中可能具有核心调节作用，本文选择８０例有随访结果的骨肉瘤，应用免疫组化方法检测 ＮＦ－кＢ ｐ６５亚单位以及血管内皮细胞生长因子（ＶＥＧＦ）的表达，同时观察肿瘤内微血管密度（ＭＶＤ），旨在探讨 ＮＦ－кＢ表达与骨肉瘤血管生成的关系。结果：８０例骨肉瘤中 ４８例 ＮＦ－кＢ ｐ６５阳性，阳性产物主要定位于肿瘤细胞浆。ＮＦ－кＢ表达与ＶＥＧＦ表达和ＭＶＤ呈显著正相关，而且ＮＦ－кＢ阳性组病人存活时间显著低于阴性组。该结果表明：①ＮＦ－кＢ ｐ６５是骨肉瘤相关的一种癌蛋白，在骨肉瘤的发生和发展中发挥重要的作用；②ＮＦ－кＢ对ＶＥＧＦ可能有正向调节作用，进而影响骨肉瘤血管生成；③ＮＦ－кＢ　对肿瘤血管生成的作用，可能是影响骨肉瘤预后的主要原因之一。The recent studies suggest that nuclear factor GB(NF-кB)may play a central role in the angiogenesis process. To probe into the relationship between NF-кB p65 subunit and vescular endothelial cell growth factor (VEGF),VEGF Was detected immunohistochemically in 80 specimans of osteosarcoma with follow-up. Meanwhile,the intratumoral rnicrovessel density (MVD ) was calculated with morplometry in them. Results:out of 80 cases of osteosarcoma,48 were positive staining for NF-кB p65 subunit. The positive product was mainly located in the cytoplasm of tumor cell. A positive relationship was found between the NF-кB expression and VEGF expression as well as the MVD. Furthermore,the, survival time was much shorter in group with positive NF-кB than in group with negative NF-кB. The results suggest that: (1 )NF-кB may beanimportant oncoprotein ，and Involved in the progress of osteosarcoma; (2)NF-кB may induce the angiogenesis by up-regulating the VEGF expression; (3)Involvement of Nc-кB in angiogenesis may be one of the most mportant factors to influence prognosis in patients with osteosarcoma.

关 键 词：骨肉瘤 细胞核因子 NF-ΚB 免疫组织化学 

分 类 号：R738.102[医药卫生—肿瘤]