人转铁蛋白—葡萄球菌肠毒素B偶联蛋白的抗肿瘤活性  

Study of Anti - Tumor Activity of Human TransferrinStaphylococcus Aureus Enterotoxin B Coupled Protein

作　　者：张国驰[1] 陶箭[1] 朱佑明[1] 童善庆[1] 陆德源[1] 赵任[2] 

机构地区：[1]上海第二医科大学微生物学教研室 [2]上海第二医科大学附属瑞金医院普外科,200025

出　　处：《上海第二医科大学学报》1999年第3期256-258,267,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：上海市免疫研究所资助!9808

摘　　要：目的研究人转铁蛋白（HuTf）与金黄色葡萄球菌肠毒素B（SEB）偶联后，其Tf－SEB偶合蛋白的抗肿瘤活性，探讨Tf作为载体用于肿瘤导向免疫治疗的可能性。方法利用凋亡细胞指数检测和超抗原依赖的细胞介导的细胞毒（SDCC）效应，观察该偶合蛋白的起抗原活性和激活T细胞杀伤肿瘤细胞的作用。结果Tf－SEB偶合蛋白比单体SEB能更有效地激活T细胞的杀伤肿瘤细胞活性，并对MHC－Ⅱ类分子阴性的肿瘤靶细胞也具有较强的杀伤效果。结论Tf作为载体应用于肿瘤的导向治疗具有可行性。Objective Study the anti- tumor activity of human transferrin (HuT f)coupled with superantigen, Staphylococcus aureus enterotoxin B(SEB), and the possibility oftransferrin - targeted in immunotherapy of human tumor. Methods Examined the super-antigen activity of Tf- SEB coupled protein and the effect of Tf- SEB to stimulate T cells tokill tumor cells. By detecting the target cell apoptotic index and the superantigen dependentcell - mediated cytotoxicity (SDCC) to examine the ability of Tf- SEB activating T cells tokill the tumor ce1l. ResuIts By comparing with the SEB, we found that Tf- SEB was moreeffect to activate T cells to kill tumor cells and even kill the MHC - II negative tumor cellsmore significant1y. ConcIusion It is possible for Tf as a carrier to be used in thecarrier - targeted therapy for tumorous diseases.

关 键 词：转铁蛋白 肠毒素B 载体 SEB 肿瘤 免疫疗法 

分 类 号：R730.51[医药卫生—肿瘤]