与肺癌和胃癌转移可能相关的RAB5A基因  

RAB5A, a gene possibly related to metastasis of human carcinoma of the lung and stomach

作　　者：李钰[1] 冯会臣[1] 陈宇[1] 邹嵘[1] 张贵寅[1] 李璞[1] 

机构地区：[1]哈尔滨医科大学医学遗传学研究室,150086

出　　处：《中华肿瘤杂志》1999年第3期178-181,共4页Chinese Journal of Oncology

基　　金：黑龙江省首届杰出青年基金;国家教委跨世纪人才基金

摘　　要：目的　研究和分离肿瘤转移相关基因，探讨肺癌及胃癌转移发生的分子基础。方法应用细胞培养、ｍＲＮＡ差异显示技术、ｃＤＮＡ 克隆、测序技术、ＲＴＰＣＲ、Ｎｏｒｔｈｅｒｎ 印迹杂交技术以及免疫组化技术，分析比较了细胞来源相同，但转移能力不同的人肺腺癌细胞系Ａｎｉｐ９７３ 和ＡＧＺＹ８３ａ 基因差异表达及其差异表达基因ＲＡＢ５Ａ在临床胃癌和肺癌标本中存在的实际意义。结果　在两细胞系之间确有明显的差异表达基因存在，ＲＡＢ５Ａ基因在具有高转移潜能的Ａｎｉｐ９７３ 中高表达。３３ 例肺癌和４７ 例胃癌样品的石蜡切片经免疫组化检测证实，ＲＡＢ５Ａ 表达程度与肺癌和胃癌的转移有关（ Ｐ＜０ ．０５） ，而且与胃癌分化程度有关。结论　ＲＡＢ５Ａ是一个可能与肺癌、胃癌转移相关的基因，并可能与胃癌的分化程度有一定关系，分化程度越低，其表达程度越高。Objective To study and isolate tumor metastasis related genes, and to explore the molecular basis of metastasis of lung and gastric carcinoma.Methods Using mRNA differential display technique, cDNA cloning and sequencing, RT PCR, Northern blot and immunocytochemical methods, genes differentially expressed between human lung adenocarcinoma cell lines AGZY83 a and Anip973 were detected. Anip973 was isolated from AGZY83 a with much higher metastatic potential than the parent line.Results There were significant differences in gene expression between the two cell lines. RAB5A was one of the genes differentially expressed in Anip973 cells. Its gene product is a GTP binding protein. Paraffin sections of 33 lung carcinoma and 47 gastric carcinoma specimens were examined immunohistochemically. Expression of RAB5A was positive in 87.9% of NSCLC and 91.5% of gastric cancer specimens examined ( P <0.05), and correlated with their metastatic potential and differentiation degree of gastric carcinoma. Conclusion RAB5A is possibly a metastasis related gene.

关 键 词：肺肿瘤 胃肿瘤 RAB5A基因 基因表达 肿瘤转移 

分 类 号：R734.2[医药卫生—肿瘤] R735.2[医药卫生—临床医学]